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Literature summary extracted from

  • Webster, G.C.
    Isolation of an alanine-activating enzyme from pig liver (1961), Biochim. Biophys. Acta, 49, 141-152.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.1.1.7 2,6-dichlorophenol-indophenol enhances activity Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.1.1.7 2-mercaptoethanol
-
 Sus scrofa
6.1.1.7 2-mercaptoethylamine
-
 Sus scrofa
6.1.1.7 L-cysteine
-
 Sus scrofa
6.1.1.7 L-glutathione
-
 Sus scrofa
6.1.1.7 p-Substituted mercuribenzoate
-
 Sus scrofa
6.1.1.7 sulfhydryl group containing substances
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.1.1.7 cytoplasm
-
 Sus scrofa 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.1.1.7 Co2+ or Mg2+ required Sus scrofa
6.1.1.7 Mg2+ or Co2+ required Sus scrofa

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7
-
 Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.1.1.7 liver
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.1.1.7 0.027
-
 alanyl-tRNA synthesis Sus scrofa
6.1.1.7 0.114
-
 diphosphate-ATP exchange Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla marked specificity for ATP. ADP, GTP, ITP, CTP and UTP are inactive Sus scrofa AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 additional information synthesis of alanyl hydroxamate with high concentrations of hydroxylamine Sus scrofa ?
-
 ?
6.1.1.7 additional information alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate Sus scrofa ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.1.1.7 7.5
-
 alanyl-tRNA synthesis Sus scrofa
6.1.1.7 8.5
-
 ATP-diphosphate exchange Sus scrofa