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Literature summary extracted from

  • Parkin, K.L.; Hultin, H.O.
    Characterisation of trimethylamine-N-oxide (TMAO) demethylase activity from fish muscle microsomes (1986), J. Biochem., 100, 77-86.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.2.32 cysteine cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions Urophycis chuss
4.1.2.32 additional information antagonistic relationship between two cofactor systems, implying competition for a single site Urophycis chuss

General Stability

EC Number General Stability Organism
4.1.2.32 inclusion of mercaptoethanol, EDTA and PMSF in the preparative buffers enhance yield and stability of microsomal activity Urophycis chuss

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.32 ascorbate activity stimulated by NADH and FMN (anaerobic) is inhibited by 40% Urophycis chuss
4.1.2.32 cyanide K-salt of inhibits both cofactor systems by 75% Urophycis chuss
4.1.2.32 FeCl2 activity stimulated by NADH and FMN (anaerobic) is inhibited by 95% in the presence of 0.2 mM FeCl2, inhibition cannot be relieved by the presence of EDTA. Activity stimulated by ascorbate is inhibited by 94% in the presence of 0.2 mM FeCl2 Urophycis chuss
4.1.2.32 FMN 30% inhibition in the presence of Fe2+, cysteine, and ascorbate Urophycis chuss
4.1.2.32 iodoacetamide pre-incubation results in 25% loss of activity in both cofactor systems Urophycis chuss
4.1.2.32 additional information overview Urophycis chuss
4.1.2.32 NaN3 100% inhibition of NADH/FMN cofactor system (anaerobic conditions), 40% inhibition of the ascorbate/cysteine/Fe2+ cofactor system under anaerobic conditions Urophycis chuss
4.1.2.32 trimethylamine
-
 Urophycis chuss

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.32 2.7
-
 Trimethylamine N-oxide catalyzed aerobically in presence of Fe2+ (0.2 mM), ascorbate (2 mM), and cysteine (2 mM) Urophycis chuss
4.1.2.32 21
-
 Trimethylamine N-oxide catalyzed anaerobically in presence of FMN (0.1 mM) and NADH (0.4 mM) Urophycis chuss

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.1.2.32 microsome
-
 Urophycis chuss
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.32 Fe2+ cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions Urophycis chuss
4.1.2.32 Fe2+ no activity when Fe2+ is replaced by the Cl-salts of Ca2+, Mn2+, Mg2+, Ni2+, Zn2+, or Co2+ for the ascorbate, cysteine, and Fe2+ cofactor system Urophycis chuss

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.32 Urophycis chuss
-
 red hake
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.2.32 muscle
-
 Urophycis chuss
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.32 Trimethylamine N-oxide
-
 Urophycis chuss Dimethylamine + formaldehyde
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.32 40
-
 activity generated by both cofactor systems is lost Urophycis chuss
4.1.2.32 100
-
 heating for 5 min deactivates partial purified fraction Urophycis chuss

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.32 ascorbate cofactor system composed of Fe2+, cysteine and/or ascorbate functions under aerobic and anaerobic conditions, a second cofactor system with NADH/FMN requires anaerobic conditions Urophycis chuss
4.1.2.32 FMN cofactor system NADH/FMN requires anaerobic conditions, a second cofactor system composed of Fe2+, cysteine and/or ascorbate under aerobic or anaerobic conditions Urophycis chuss
4.1.2.32 NADH cofactor system NADH/FMN requires anaerobic conditions, a second cofactor system composed of Fe2+, cysteine and/or ascorbate under aerobic or anaerobic conditions Urophycis chuss