EC Number | General Stability | Organism |
---|---|---|
4.1.2.32 | enzyme is unstable when purified more than about 5-fold | Aminobacter aminovorans |
4.1.2.32 | exposure of the enzyme for 30 min at 0°C to buffers of pH values in the region 4 to 9 does not result in loss of activity followed by adjustment of the pH 6.0 | Aminobacter aminovorans |
4.1.2.32 | trimethylamine-grown Pseudomonas aminovorans enzyme is more labile at 40°C than enzyme from methylamine-grown bacteria in French press extracts | Aminobacter aminovorans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.2.32 | cyanide | active at 0.001 mM | Aminobacter aminovorans | |
4.1.2.32 | Mercurials | active at 0.001 mM | Aminobacter aminovorans | |
4.1.2.32 | Triethylamine N-oxide | - |
Aminobacter aminovorans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.2.32 | 2 | - |
Trimethylamine N-oxide | - |
Aminobacter aminovorans | |
4.1.2.32 | 140 | - |
Benzyldimethylamine N-oxide | - |
Aminobacter aminovorans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.32 | additional information | Aminobacter aminovorans | enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.2.32 | Aminobacter aminovorans | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.2.32 | partial | Aminobacter aminovorans |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
4.1.2.32 | trimethylamine N-oxide = dimethylamine + formaldehyde | non-oxidative, non-hydrolytic cleavage | Aminobacter aminovorans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.2.32 | Benzyldimethylamine N-oxide | 50% of the activity with trimethylamine N-oxide | Aminobacter aminovorans | ? | - |
? | |
4.1.2.32 | additional information | enzyme is present in cells grown on methylamine, dimethylamine, trimethylamine and trimethyl N-oxide and is induced before growth begins when succinate-grown Pseudomonas aminovorans is transferred to an amine growth medium | Aminobacter aminovorans | ? | - |
? | |
4.1.2.32 | Trimethylamine N-oxide | - |
Aminobacter aminovorans | Dimethylamine + formaldehyde | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.2.32 | 25 | - |
assay at | Aminobacter aminovorans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.32 | 6 | - |
- |
Aminobacter aminovorans |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
4.1.2.32 | 5 | 7.5 | pH 5.0: about 75% of maximal activity, pH 7.5: about 75% of maximal activity | Aminobacter aminovorans |