BRENDA - Enzyme Database show

Kinetic analysis of duodenal and testicular cytochrome P450c17 in the rat

Dalla Valle, L.; Ramina, A.; Vianello, S.; Belvedere, P.; Colombo, L.; J. Steroid Biochem. Mol. Biol. 58, 577-584 (1996)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.19
estradiol-17beta
inhibits both and C17,20 lyase activity in testis and duodenum, competitive inhibitor
Rattus norvegicus
1.14.14.32
estradiol-17beta
concentration of 0.3 mM both, testicular and male duodenal enzyme with exogenous 17alpha-hydroxyprogesterone as substrate are inhibited by 30-40%
Rattus norvegicus
1.14.14.32
ketoconazole
competitve
Rattus norvegicus
1.14.14.32
Promegestone
competitive
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.19
0.0000142
-
progesterone
17alpha-hydroxylation in testis
Rattus norvegicus
1.14.14.19
0.0000232
-
progesterone
17alpha-hydroxylation in female duodenum
Rattus norvegicus
1.14.14.19
0.0000238
-
progesterone
17alpha-hydroxylation in male duodenum
Rattus norvegicus
1.14.14.19
0.000525
-
17alpha-hydroxyprogesterone
C17,20 lyase activity in testis
Rattus norvegicus
1.14.14.19
0.000637
-
17alpha-hydroxyprogesterone
C17,20 lyase activity in female duodenum
Rattus norvegicus
1.14.14.19
0.000675
-
17alpha-hydroxyprogesterone
C17,20 lyase activity in male duodenum
Rattus norvegicus
1.14.14.32
0.000015
-
17alpha-hydroxyprogesterone
intermediate substrate, testis enzyme
Rattus norvegicus
1.14.14.32
0.0000248
-
17alpha-hydroxyprogesterone
intermediate substrate, female duodenum enzyme
Rattus norvegicus
1.14.14.32
0.0000314
-
17alpha-hydroxyprogesterone
intermediate substrate, male duodenum enzyme
Rattus norvegicus
1.14.14.32
0.000525
-
17alpha-hydroxyprogesterone
exogenous substrate, testis enzyme
Rattus norvegicus
1.14.14.32
0.000637
-
17alpha-hydroxyprogesterone
exogenous substrate, female duodenum enzyme
Rattus norvegicus
1.14.14.32
0.000675
-
17alpha-hydroxyprogesterone
exogenous substrate, male duodenum enzyme
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.19
microsome
-
Rattus norvegicus
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.14.19
Fe3+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.19
pregnenolone + AH2 + O2
Rattus norvegicus
-
17alpha-hydroxypregnenolone + A + H2O
product eliminates at C20,21 acetate to yield dehydroepiandrosterone
Rattus norvegicus
?
1.14.14.19
progesterone + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
-
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
product eliminates at C20,21 acetate to yield androstenedione
Rattus norvegicus
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.14.14.19
Rattus norvegicus
-
-
-
1.14.14.32
Rattus norvegicus
-
Wistar rat
-
1.14.14.32
vertebrata
-
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.19
duodenum
-
Rattus norvegicus
-
1.14.14.19
testis
-
Rattus norvegicus
-
1.14.14.32
duodenum
-
Rattus norvegicus
-
1.14.14.32
gonad
-
Rattus norvegicus
-
1.14.14.32
gonad
-
vertebrata
-
1.14.14.32
liver
-
Rattus norvegicus
-
1.14.14.32
placenta
-
Rattus norvegicus
-
1.14.14.32
stomach
-
Rattus norvegicus
-
1.14.14.32
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.19
pregnenolone + AH2 + O2
-
5129
Rattus norvegicus
17alpha-hydroxypregnenolone + A + H2O
product eliminates at C20,21 acetate to yield dehydroepiandrosterone
5129
Rattus norvegicus
?
1.14.14.19
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
5129
Rattus norvegicus
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
product eliminates at C20,21 acetate to yield androstenedione
5129
Rattus norvegicus
?
1.14.14.32
17alpha-Hydroxypregnenolone
-
5129
Rattus norvegicus
Dehydroepiandrosterone + acetaldehyde
-
-
-
-
1.14.14.32
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
5129
Rattus norvegicus
androstenedione + acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
5129
Rattus norvegicus
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.19
37
-
-
Rattus norvegicus
1.14.14.32
37
-
assay at
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.19
NADPH
-
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.14.14.19
NADPH
-
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.14.14.19
estradiol-17beta
inhibits both and C17,20 lyase activity in testis and duodenum, competitive inhibitor
Rattus norvegicus
1.14.14.32
estradiol-17beta
concentration of 0.3 mM both, testicular and male duodenal enzyme with exogenous 17alpha-hydroxyprogesterone as substrate are inhibited by 30-40%
Rattus norvegicus
1.14.14.32
ketoconazole
competitve
Rattus norvegicus
1.14.14.32
Promegestone
competitive
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.14.14.19
0.0000142
-
progesterone
17alpha-hydroxylation in testis
Rattus norvegicus
1.14.14.19
0.0000232
-
progesterone
17alpha-hydroxylation in female duodenum
Rattus norvegicus
1.14.14.19
0.0000238
-
progesterone
17alpha-hydroxylation in male duodenum
Rattus norvegicus
1.14.14.19
0.000525
-
17alpha-hydroxyprogesterone
C17,20 lyase activity in testis
Rattus norvegicus
1.14.14.19
0.000637
-
17alpha-hydroxyprogesterone
C17,20 lyase activity in female duodenum
Rattus norvegicus
1.14.14.19
0.000675
-
17alpha-hydroxyprogesterone
C17,20 lyase activity in male duodenum
Rattus norvegicus
1.14.14.32
0.000015
-
17alpha-hydroxyprogesterone
intermediate substrate, testis enzyme
Rattus norvegicus
1.14.14.32
0.0000248
-
17alpha-hydroxyprogesterone
intermediate substrate, female duodenum enzyme
Rattus norvegicus
1.14.14.32
0.0000314
-
17alpha-hydroxyprogesterone
intermediate substrate, male duodenum enzyme
Rattus norvegicus
1.14.14.32
0.000525
-
17alpha-hydroxyprogesterone
exogenous substrate, testis enzyme
Rattus norvegicus
1.14.14.32
0.000637
-
17alpha-hydroxyprogesterone
exogenous substrate, female duodenum enzyme
Rattus norvegicus
1.14.14.32
0.000675
-
17alpha-hydroxyprogesterone
exogenous substrate, male duodenum enzyme
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.14.14.19
microsome
-
Rattus norvegicus
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.14.14.19
Fe3+
-
Rattus norvegicus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.14.14.19
pregnenolone + AH2 + O2
Rattus norvegicus
-
17alpha-hydroxypregnenolone + A + H2O
product eliminates at C20,21 acetate to yield dehydroepiandrosterone
Rattus norvegicus
?
1.14.14.19
progesterone + [reduced NADPH-hemoprotein reductase] + O2
Rattus norvegicus
-
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
product eliminates at C20,21 acetate to yield androstenedione
Rattus norvegicus
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.14.14.19
duodenum
-
Rattus norvegicus
-
1.14.14.19
testis
-
Rattus norvegicus
-
1.14.14.32
duodenum
-
Rattus norvegicus
-
1.14.14.32
gonad
-
Rattus norvegicus
-
1.14.14.32
gonad
-
vertebrata
-
1.14.14.32
liver
-
Rattus norvegicus
-
1.14.14.32
placenta
-
Rattus norvegicus
-
1.14.14.32
stomach
-
Rattus norvegicus
-
1.14.14.32
testis
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.14.14.19
pregnenolone + AH2 + O2
-
5129
Rattus norvegicus
17alpha-hydroxypregnenolone + A + H2O
product eliminates at C20,21 acetate to yield dehydroepiandrosterone
5129
Rattus norvegicus
?
1.14.14.19
progesterone + [reduced NADPH-hemoprotein reductase] + O2
-
5129
Rattus norvegicus
17alpha-hydroxyprogesterone + [oxidized NADPH-hemoprotein reductase] + H2O
product eliminates at C20,21 acetate to yield androstenedione
5129
Rattus norvegicus
?
1.14.14.32
17alpha-Hydroxypregnenolone
-
5129
Rattus norvegicus
Dehydroepiandrosterone + acetaldehyde
-
-
-
-
1.14.14.32
17alpha-hydroxyprogesterone + [reduced NADPH-hemoprotein reductase] + O2
-
5129
Rattus norvegicus
androstenedione + acetate + [oxidized NADPH-hemoprotein reductase] + H2O
-
5129
Rattus norvegicus
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.14.14.19
37
-
-
Rattus norvegicus
1.14.14.32
37
-
assay at
Rattus norvegicus