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Literature summary extracted from

  • Lechler, A.; Martin, A.; Zuleeg, T.; Limmer, S.; Kreutzer, R.
    A biologically active 53 kDa fragment of overproduced alanyl-tRNA synthetase from Thermus thermophilus HB8 specifically interacts with tRNAAla acceptor helix (1997), Nucleic Acids Res., 25, 2737-2744.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.1.1.7 expression in Escherichia coli Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.1.1.7 205000
-
 size exclusion chromatography Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.7 Thermus thermophilus P74941 overproduced in Escherichia coli
-
6.1.1.7 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 P74941 overproduced in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.7
-
 Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Thermus thermophilus AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 ATP + L-alanine + tRNAAla
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 AMP + diphosphate + L-alanyl-tRNAAla
-
 ?
6.1.1.7 additional information alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate Thermus thermophilus ?
-
 ?
6.1.1.7 additional information alanine dependent ATP-diphosphate exchange: alanine + ATP + enzyme /alanine-AMP-enzyme + diphosphate Thermus thermophilus HB8 / ATCC 27634 / DSM 579 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.1.1.7 60
-
 aminoacylation Thermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.1.1.7 65
-
 stable up to Thermus thermophilus
6.1.1.7 85
-
 denaturation at Thermus thermophilus