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Literature summary extracted from

  • Fish, D.C.; Blumenthal, H.J.
    2-keto-3-deoxy-D-Glucarate aldolase (1966), Methods Enzymol., 9, 529-534.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.20 CN- reversed by Mg2+ and Co2+, but not by Fe2+ Escherichia coli
4.1.2.20 diphosphate
-
 Escherichia coli
4.1.2.20 EDTA
-
 Escherichia coli
4.1.2.20 F-
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.20 additional information
-
 additional information
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.20 Co2+ absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ Escherichia coli
4.1.2.20 Fe2+ absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ Escherichia coli
4.1.2.20 Mg2+ absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ Escherichia coli
4.1.2.20 Mn2+ absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ Escherichia coli
4.1.2.20 Mo2+ absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.20 Escherichia coli
-
-
-
4.1.2.20 Escherichia coli CR63MA
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.20
-
 Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.20 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.20 2-dehydro-3-deoxy-D-glucarate
-
 Escherichia coli pyruvate + tartronate semialdehyde
-
 ?
4.1.2.20 2-dehydro-3-deoxy-D-glucarate
-
 Escherichia coli CR63MA pyruvate + tartronate semialdehyde
-
 ?
4.1.2.20 5-dehydro-4-deoxy-D-glucarate
-
 Escherichia coli pyruvate + tartronate semialdehyde
-
 ?
4.1.2.20 5-dehydro-4-deoxy-D-glucarate
-
 Escherichia coli CR63MA pyruvate + tartronate semialdehyde
-
 ?
4.1.2.20 pyruvate + glycolaldehyde
-
 Escherichia coli 2-oxo-4,5-dihydroxy-L-pentanoic acid
-
 ?
4.1.2.20 pyruvate + glycolaldehyde
-
 Escherichia coli CR63MA 2-oxo-4,5-dihydroxy-L-pentanoic acid
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.20 7.7 8.6
-
 Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.20 6 7.5 stable Escherichia coli