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Literature summary extracted from
- 2-Keto-3-deoxy-6-phosphogluconic and related aldolases (1972), The Enzymes, 3rd Ed. (Boyer, P. D. , ed. ), 7, 281-302.
No PubMed abstract available
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 4.1.2.14 | highly stable when frozen in phosphate buffer | Pseudomonas putida |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.14 | 2-Keto-3-deoxy-6-phosphogalaconate | - |
Pseudomonas putida |  |
| 4.1.2.14 | 2-keto-3-deoxygluconate | - |
Pseudomonas putida | |
| 4.1.2.14 | 5-Keto-4-deoxyglucarate | - |
Pseudomonas putida | |
| 4.1.3.17 | 2-oxoglutarate | - |
Arachis hypogaea | |
| 4.1.3.17 | pyruvate | - |
Arachis hypogaea | |
| 4.1.3.42 | pyruvate | competitive inhibition of glyoxylate binding | Bacteria | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.14 | 0.1 | - |
6-phospho-2-dehydro-3-deoxy-D-gluconate | - |
Pseudomonas putida | |
| 4.1.2.23 | 6 | - |
3-deoxy-D-manno-octulosonate | - |
Enterobacter cloacae | |
| 4.1.3.17 | 0.086 | - |
4-hydroxy-4-methyl-2-oxoglutarate | - |
Arachis hypogaea | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.2.20 | Co2+ | absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ | Escherichia coli | |
| 4.1.2.20 | Fe2+ | absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ | Escherichia coli | |
| 4.1.2.20 | Mg2+ | absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ | Escherichia coli | |
| 4.1.2.20 | Mn2+ | absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ | Escherichia coli | |
| 4.1.2.20 | Mo2+ | absolute cation requirement, activation in the order of increasing effectiveness: Mg2+, Co2+, Fe2+, Mn2+, Mo2+ | Escherichia coli | |
| 4.1.3.42 | additional information | no requirement | Bacteria |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.14 | 72000 | 78000 | disc gel electrophoresis | Pseudomonas putida |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.20 | additional information | Escherichia coli | inducible enzyme | ? | - |
? | |
| 4.1.3.42 | 4-hydroxy-2-oxoglutarate | Bacteria | catabolism of hydroxyproline, condensation physiologically less important | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.2.14 | Pelomonas saccharophila | - |
- |
- |
| 4.1.2.14 | Pseudomonas putida | - |
- |
- |
| 4.1.2.20 | Escherichia coli | - |
- |
- |
| 4.1.2.20 | no activity in Bacillus subtilis | - |
hexarate-grown | - |
| 4.1.2.21 | Pelomonas saccharophila | - |
- |
- |
| 4.1.2.23 | Enterobacter cloacae | - |
- |
- |
| 4.1.3.17 | Arachis hypogaea | - |
- |
- |
| 4.1.3.42 | Bacteria | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.2.23 | - |
Enterobacter cloacae |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate | mechanism | Pseudomonas putida | |
| 4.1.2.14 | 2-dehydro-3-deoxy-6-phospho-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate | Schiff base formation between a lysine epsilon-amino group and carbonyl compounds 6-phospho-2-dehydro-3-deoxy-D-gluconate | Pseudomonas putida |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.3.17 | cotyledon | - |
Arachis hypogaea | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.23 | additional information | - |
- |
Enterobacter cloacae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.2.14 | 2-dehydro-3-deoxy-D-gluconate 6-phosphate | - |
Pelomonas saccharophila | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
| 4.1.2.14 | 2-dehydro-3-deoxy-D-gluconate 6-phosphate | highly specific in direction of cleavage | Pseudomonas putida | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
| 4.1.2.14 | additional information | catalyzes decarboxylation of oxaloacetate at much slower rate than it cleaves | Pseudomonas putida | ? | - |
? | |
| 4.1.2.14 | additional information | catalyzes exchange of the methyl hydrogen of pyruvate with protons of water | Pseudomonas putida | ? | - |
? | |
| 4.1.2.14 | additional information | catalyzes exchange of the methyl hydrogen of pyruvate with protons of water | Pelomonas saccharophila | ? | - |
? | |
| 4.1.2.20 | 2-dehydro-3-deoxy-D-glucarate | - |
Escherichia coli | pyruvate + tartronate semialdehyde | - |
? | |
| 4.1.2.20 | additional information | inducible enzyme | Escherichia coli | ? | - |
? | |
| 4.1.2.20 | Pyruvate + D-glyceraldehyde | - |
Escherichia coli | ? | - |
? | |
| 4.1.2.20 | pyruvate + glycolaldehyde | - |
Escherichia coli | 2-oxo-4,5-dihydroxy-L-pentanoic acid | - |
? | |
| 4.1.2.20 | Pyruvate + glyoxylate | - |
Escherichia coli | ? | - |
? | |
| 4.1.2.20 | Pyruvate + L-glyceraldehyde | - |
Escherichia coli | ? | - |
? | |
| 4.1.2.20 | pyruvate + tartronate semialdehyde | - |
Escherichia coli | 2-dehydro-3-deoxy-D-glucarate | - |
? | |
| 4.1.2.20 | pyruvate + tartronate semialdehyde | - |
Escherichia coli | 5-dehydro-4-deoxy-D-glucarate | - |
? | |
| 4.1.2.21 | 2-dehydro-3-deoxy-D-galactonate 6-phosphate | - |
Pelomonas saccharophila | pyruvate + D-glyceraldehyde 3-phosphate | - |
? | |
| 4.1.2.23 | 3-deoxy-D-manno-octulosonate | - |
Enterobacter cloacae | pyruvate + D-arabinose | - |
? | |
| 4.1.2.23 | pyruvate + D-arabinose | - |
Enterobacter cloacae | 3-deoxy-D-manno-octulosonate | - |
? | |
| 4.1.2.23 | pyruvate + D-ribose | - |
Enterobacter cloacae | ? | - |
? | |
| 4.1.3.17 | 4-hydroxy-4-methyl-2-oxoglutarate | - |
Arachis hypogaea | pyruvate + pyruvate | - |
? | |
| 4.1.3.42 | 4-hydroxy-2-oxoglutarate | catabolism of hydroxyproline, condensation physiologically less important | Bacteria | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.2.14 | trimer | 3 * 23000-24000, SDS-PAGE | Pseudomonas putida |
| 4.1.3.42 | dimer | - |
Bacteria |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.14 | 9 | - |
inactivation above | Pseudomonas putida |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.2.14 | additional information | - |
additional information | - |
Pseudomonas putida |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.2.14 | 7.5 | 8.7 | - |
Pseudomonas putida |
| 4.1.2.23 | 7 | - |
- |
Enterobacter cloacae |
| 4.1.3.17 | 9 | - |
and above | Arachis hypogaea |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.42 | 7.4 | 9.6 | - |
Bacteria |
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