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Literature summary extracted from

  • Kusakabe, T.; Motoki, K.; Hori, K.
    Human aldolase C: characterization of the recombinant enzyme expressed in Escherichia coli (1994), J. Biochem., 115, 1172-1177.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.13 aldolase C, expression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.13 Y363S Y363S has reduced catalytic activity towards D-fructose 1,6-bisphosphate and fructose 1-phosphate Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.13 0.0027
-
 D-fructose 1,6-bisphosphate mutant aldolase C Y363S Homo sapiens
4.1.2.13 0.0089
-
 D-fructose 1,6-bisphosphate aldolase C Rattus norvegicus
4.1.2.13 0.012
-
 D-fructose 1,6-bisphosphate aldolase B Homo sapiens
4.1.2.13 0.013
-
 D-fructose 1,6-bisphosphate aldolase C Homo sapiens
4.1.2.13 0.052
-
 D-fructose 1,6-bisphosphate aldolase A Homo sapiens
4.1.2.13 1.7
-
 fructose 1-phosphate mutant aldolase C Y363S Homo sapiens
4.1.2.13 3.7
-
 fructose 1-phosphate aldolase B Homo sapiens
4.1.2.13 18
-
 fructose 1-phosphate aldolase C Homo sapiens
4.1.2.13 25
-
 fructose 1-phosphate aldolase C Rattus norvegicus
4.1.2.13 27
-
 fructose 1-phosphate aldolase A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.13 Homo sapiens
-
-
-
4.1.2.13 Rattus norvegicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Homo sapiens glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-fructose 1,6-bisphosphate
-
 Rattus norvegicus glycerone phosphate + D-glyceraldehyde 3-phosphate
-
 ?
4.1.2.13 D-Fructose 1-phosphate
-
 Rattus norvegicus Glycerone phosphate + D-glyceraldehyde
-
 r
4.1.2.13 D-Fructose 1-phosphate recombinant enzyme: 7.4% of the activity with fructose 1,6-diphosphate Homo sapiens Glycerone phosphate + D-glyceraldehyde
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.13 50
-
 30 min, about 80% loss of aldolase C activity with fructose 1-phosphate as substrate Homo sapiens
4.1.2.13 50
-
 30 min, about 70% loss of aldolase B activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens
4.1.2.13 50
-
 30 min, about 5% loss of aldolase A activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens
4.1.2.13 50
-
 30 min, about 40% loss of aldolase C activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens
4.1.2.13 60
-
 30 min, about 90% loss of aldolase A activity with D-fructose 1,6-bisphosphate as substrate Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.13 0.75
-
 fructose 1-phosphate mutant aldolase C Y363S Homo sapiens
4.1.2.13 1.28
-
 fructose 1-phosphate aldolase A Homo sapiens
4.1.2.13 1.48
-
 fructose 1-phosphate aldolase C Homo sapiens
4.1.2.13 2.67
-
 fructose 1-phosphate aldolase C Rattus norvegicus
4.1.2.13 2.83
-
 D-fructose 1,6-bisphosphate mutant aldolase C Y363S Homo sapiens
4.1.2.13 10.8
-
 fructose 1-phosphate aldolase B Homo sapiens
4.1.2.13 12.6
-
 D-fructose 1,6-bisphosphate aldolase C Homo sapiens
4.1.2.13 20.1
-
 D-fructose 1,6-bisphosphate aldolase B Homo sapiens
4.1.2.13 33
-
 D-fructose 1,6-bisphosphate aldolase C Rattus norvegicus
4.1.2.13 59.7
-
 D-fructose 1,6-bisphosphate aldolase A Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.13 5 9
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.2.13 4 12 pH 4: about 80% of maximal activity, pH 12: about 75% of maximal activity Homo sapiens