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Literature summary extracted from

  • Schaaf, P.M.M.; Heide, L.E.; Leistner, E.W.; Tani, Y.; El-Olemy, M.M.
    Immobilization of isochorismate hydroxymutase. Comparison of native versus immobilized enzyme (1993), J. Nat. Prod., 56, 1304-1312.
    View publication on PubMed

General Stability

EC Number General Stability Organism
5.4.4.2 stability of the enzyme is greatly increased by immobilization Flavobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.4.4.2 0.076
-
 isochorismate enzyme immobilized on alkylamine glass Flavobacterium sp.
5.4.4.2 0.171
-
 isochorismate enzyme immobilized on CNBr-Sepharose Flavobacterium sp.
5.4.4.2 0.254
-
 isochorismate soluble enzyme Flavobacterium sp.
5.4.4.2 0.27
-
 chorismate enzyme immobilized on CNBr-Sepharose Flavobacterium sp.
5.4.4.2 0.313
-
 chorismate enzyme immobilized on alkylamine glass Flavobacterium sp.
5.4.4.2 0.35
-
 chorismate soluble enzyme Flavobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.4.4.2 Mg2+ absolute requirement Flavobacterium sp.
5.4.4.2 Mg2+ optimal concentration for soluble enzyme: 5-20 mM. Optimal concentration for enzyme immobilized on CNBr-Sepharose or alkylamine glass: 2.5-5 mM. Activity of the soluble enzyme without Mg2+ is 5.5% of the activity without Mg2+ Flavobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
5.4.4.2 Flavobacterium sp.
-
 K3-15
-
5.4.4.2 Flavobacterium sp. K3-15
-
 K3-15
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.4.4.2 Chorismate r Flavobacterium sp. Isochorismate
-
 ?
5.4.4.2 Chorismate r Flavobacterium sp. K3-15 Isochorismate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.4.4.2 42
-
 soluble enzyme Flavobacterium sp.
5.4.4.2 60
-
 enzyme immobilized on CNBr-Sepharose or alkylamine glass Flavobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.4.4.2 4
-
 half-life of the enzyme immobilized on alkylamine glass: 210 days. Half-life of enzyme immobilized on CNBr Sepharose: 110 days Flavobacterium sp.
5.4.4.2 37
-
 40 h, immobilized enzyme, stable Flavobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4.4.2 7.4 8.5 enzyme immobilized on alkylamine glass Flavobacterium sp.
5.4.4.2 7.6 8.2 enzyme immobilized on CNBr-Sepharose Flavobacterium sp.
5.4.4.2 8
-
 soluble enzyme Flavobacterium sp.