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Literature summary extracted from

  • Shen-Miller, J.; West, C.A.
    Distribution and ent-kaurene synthetase in Helianthus annuus and Marah macrocarpus (1985), Phytochemistry, 24, 461-464.
No PubMed abstract available

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate Helianthus annuus involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
?
4.2.3.19 ent-copalyl diphosphate Marah macrocarpa involved in biosynthesis of gibberellins ent-kaurene + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.19 Helianthus annuus
-
-
-
4.2.3.19 Marah macrocarpa
-
-
-
5.5.1.13 Helianthus annuus
-
sunflower
-
5.5.1.13 Marah macrocarpa
-
wild cucumber
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.5.1.13 partial, using ammonium sulfate fractionation Helianthus annuus
5.5.1.13 partial, using ammonium sulfate fractionation Marah macrocarpa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.19 root perennial: very low activity Helianthus annuus
-
4.2.3.19 root perennial: very low activity Marah macrocarpa
-
4.2.3.19 seed
-
Marah macrocarpa
-
4.2.3.19 seedling cotyledons contain over 90% of activity Helianthus annuus
-
5.5.1.13 cotyledon
-
Helianthus annuus
-
5.5.1.13 cotyledon
-
Marah macrocarpa
-
5.5.1.13 hypocotyl
-
Helianthus annuus
-
5.5.1.13 hypocotyl
-
Marah macrocarpa
-
5.5.1.13 root
-
Helianthus annuus
-
5.5.1.13 root
-
Marah macrocarpa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.5.1.13 additional information
-
specific activity per mg of protein for different plant organs Helianthus annuus
5.5.1.13 additional information
-
specific activity per mg of protein for different plant organs Marah macrocarpa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.19 ent-copalyl diphosphate
-
Helianthus annuus ent-kaurene + diphosphate
-
?
4.2.3.19 ent-copalyl diphosphate
-
Marah macrocarpa ent-kaurene + diphosphate
-
?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Helianthus annuus ent-kaurene + diphosphate
-
?
4.2.3.19 ent-copalyl diphosphate involved in biosynthesis of gibberellins Marah macrocarpa ent-kaurene + diphosphate
-
?
5.5.1.13 copalyl diphosphate
-
Helianthus annuus ent-kaurene + diphosphate
-
?
5.5.1.13 copalyl diphosphate
-
Marah macrocarpa ent-kaurene + diphosphate
-
?
5.5.1.13 geranylgeranyl diphosphate
-
Helianthus annuus ent-kaurene + ?
-
?
5.5.1.13 geranylgeranyl diphosphate
-
Marah macrocarpa ent-kaurene + ?
-
?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Helianthus annuus
4.2.3.19 More part of bifunctional enzyme catalyzing the two step reaction from trans-geranylgeranyl-diphosphate to ent-kaurene, first step is catalyzed by EC 5.5.1.13 (ent-kaurene synthase A) Marah macrocarpa