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Literature summary extracted from

  • Nikolaropoulos, S.; Sotiroudis, T.G.
    Phosphorylase kinase from chicken gizzard. Partial purification and characterization (1985), Eur. J. Biochem., 151, 467-473.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 additional information no autoactivation Gallus gallus
2.7.11.19 additional information the nonactivated enzyme is activated either by limited proteolysis Gallus gallus
2.7.11.19 Trypsin proteolytic activation of nonactivated enzyme Gallus gallus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 EGTA
-
 Gallus gallus
2.7.11.19 phosphorylase b high concentration Gallus gallus
2.7.11.19 quercetin ATP does not reverse Gallus gallus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 additional information
-
 additional information
-
 Gallus gallus
2.7.11.19 0.45
-
 ATP
-
 Gallus gallus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ca2+ requirement Gallus gallus
2.7.11.19 Mg2+ requirement Gallus gallus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Gallus gallus
2.7.11.19 phosphate requirement, phosphate containing enzyme Gallus gallus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Gallus gallus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 to near homogeneity Gallus gallus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 gizzard smooth muscle
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 0.53
-
-
 Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Gallus gallus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b rabbit skeletal muscle Gallus gallus ADP + phosphorylase a
-
 ?
2.7.11.19 additional information troponin (whole complex) and histone IIAS are no substrates Gallus gallus ?
-
 ?
2.7.11.19 additional information phosvitin is no substrate Gallus gallus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Gallus gallus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 additional information
-
 pH 6.8/8.2 activity ratios: 0.58 Gallus gallus
2.7.11.19 9.3
-
-
 Gallus gallus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.19 6.2 9.3 progressive increase of activity from 10% to 100% of maximal activity, with half-maximal activity at pH 7 Gallus gallus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.19 Calmodulin additional activation Gallus gallus