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Literature summary extracted from

  • Pocinwong, S.; Blum, H.; Malencik, D.; Fisher, E.H.
    Phosphorylase kinase from dogfish skeletal muscle. Purification and properties (1981), Biochemistry, 20, 7219-7226.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 Catalytic subunit of cAMP-dependent protein kinase not Oryctolagus cuniculus
2.7.11.19 Catalytic subunit of cAMP-dependent protein kinase not Squalus acanthias
2.7.11.19 glycogen no significant effect on dogfish enzyme Squalus acanthias
2.7.11.19 additional information no activation by glucose 6-phosphate, UDPglucose, dogfish myosin, actin, tropomyosin, rabbit glycogen synthase Oryctolagus cuniculus
2.7.11.19 additional information no activation by glucose 6-phosphate, UDPglucose, dogfish myosin, actin, tropomyosin, rabbit glycogen synthase Squalus acanthias
2.7.11.19 additional information No activation by substrates of phosphorylase b reaction, i.e. AMP or glucose 1-phosphate Oryctolagus cuniculus
2.7.11.19 additional information No activation by substrates of phosphorylase b reaction, i.e. AMP or glucose 1-phosphate Squalus acanthias
2.7.11.19 additional information no activation by parvalbumin Oryctolagus cuniculus
2.7.11.19 additional information no activation by parvalbumin Squalus acanthias
2.7.11.19 Protein kinases not Squalus acanthias

General Stability

EC Number General Stability Organism
2.7.11.19 Ca2+-free enzyme is unstable Squalus acanthias
2.7.11.19 Rapid irreversible inactivation during desalting by gel filtration or dialysis at ionic strength below 0.1, sucrose, glycerol, salts, SH-compounds or reagents does not stabilize, glycogen, glucose, glucose 1-phosphate, glucose 6-phosphate, mono-, di- and trinucleotides or divalent metal ions and protease inhibitors do not protect Squalus acanthias
2.7.11.19 Tends to aggregate upon standing Squalus acanthias

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 EDTA Ca2+ restores Oryctolagus cuniculus
2.7.11.19 EDTA Ca2+ restores Squalus acanthias
2.7.11.19 EGTA Ca2+ restores; strong Oryctolagus cuniculus
2.7.11.19 EGTA Ca2+ restores; strong Squalus acanthias
2.7.11.19 Protein phosphatase rabbit (not dogfish) kinase Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 0.084
-
 phosphorylase b from rabbit, pH 8.2, dogfish enzyme Squalus acanthias
2.7.11.19 0.1
-
 MgATP2- dogfish phosphorylase b, pH 8.2, dogfish enzyme Squalus acanthias

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.19 cytosol
-
 Oryctolagus cuniculus 5829
-
2.7.11.19 cytosol
-
 Squalus acanthias 5829
-
2.7.11.19 soluble
-
 Oryctolagus cuniculus
-
-
2.7.11.19 soluble
-
 Squalus acanthias
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ba2+
-
 Oryctolagus cuniculus
2.7.11.19 Ba2+ activation, can replace Ca2+ with 26% efficiency Squalus acanthias
2.7.11.19 Ca2+
-
 Oryctolagus cuniculus
2.7.11.19 Ca2+ requirement Squalus acanthias
2.7.11.19 Co2+ activation, can replace Ca2+ with 10% efficiency Squalus acanthias
2.7.11.19 Fe3+ activation, can replace Ca2+ with 10% efficiency Squalus acanthias
2.7.11.19 Li+ activation Oryctolagus cuniculus
2.7.11.19 Li+ activation Squalus acanthias
2.7.11.19 Li+ can replace Ca2+ with 10% efficiency Squalus acanthias
2.7.11.19 Mg2+ requirement Oryctolagus cuniculus
2.7.11.19 Mg2+ requirement Squalus acanthias
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Squalus acanthias
2.7.11.19 Mn2+ requirement Oryctolagus cuniculus
2.7.11.19 Mn2+ requirement Squalus acanthias
2.7.11.19 Mn2+ can replace Ca2+ with 15% efficiency Oryctolagus cuniculus
2.7.11.19 Mn2+ can replace Ca2+ with 15% efficiency Squalus acanthias
2.7.11.19 additional information no activation by Cu2+, Cd2+, Sn2+, Al3+ Oryctolagus cuniculus
2.7.11.19 additional information no activation by Cu2+, Cd2+, Sn2+, Al3+ Squalus acanthias
2.7.11.19 additional information Fe2+, Zn2+ or Ni2+ Oryctolagus cuniculus
2.7.11.19 additional information Fe2+, Zn2+ or Ni2+ Squalus acanthias
2.7.11.19 phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Squalus acanthias
2.7.11.19 Sr2+ activation, can replace Ca2+ with 45% efficiency Oryctolagus cuniculus
2.7.11.19 Sr2+ activation, can replace Ca2+ with 45% efficiency Squalus acanthias

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 1300000
-
 dogfish, gel filtration or sedimentation velocity analysis Oryctolagus cuniculus
2.7.11.19 1300000
-
 dogfish, gel filtration or sedimentation velocity analysis Squalus acanthias

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-
2.7.11.19 Squalus acanthias
-
 i.e. Pacific dogfish or Squalus acanthias
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19
-
 Squalus acanthias

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 additional information
-
 Oryctolagus cuniculus
-
2.7.11.19 additional information tissue distribution in dogfish Squalus acanthias
-
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-
2.7.11.19 skeletal muscle
-
 Squalus acanthias
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 2.8
-
 dogfish Squalus acanthias

Storage Stability

EC Number Storage Stability Organism
2.7.11.19 -15°C, 3 mg dogfish enzyme/ml, in 0.1 M glycerophosphate, 2 mM EDTA, pH 7, 0.3 M NaCl or in 0.1 M glycerophosphate, 2 mM EDTA, 10% sucrose, 1 mM ATP, pH 7, 1 mM DTT, about 50% loss of activity within 1 week Squalus acanthias
2.7.11.19 -15°C, partially purified dogfish enzyme, lyophilized, almost indefinitely Squalus acanthias

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + nonactivated phosphorylase kinase i.e. autophosphorylation and autoactivation Oryctolagus cuniculus ADP + activated phosphorylase kinase
-
 ?
2.7.11.19 ATP + nonactivated phosphorylase kinase i.e. autophosphorylation and autoactivation Squalus acanthias ADP + activated phosphorylase kinase
-
 ?
2.7.11.19 ATP + nonactivated phosphorylase kinase ATP can be replaced by dATP or adenosine 5'-(3-thiotriphosphate) with 50% and 10% efficiency, respectively Squalus acanthias ADP + activated phosphorylase kinase
-
 ?
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Squalus acanthias ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b dogfish Squalus acanthias ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b rabbit skeletal muscle Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b rabbit skeletal muscle Squalus acanthias ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + synthetic tetradecapeptide i.e. Ser-Asp-Gln-Glu-Lys-Arg-Lys-Gln-Ile-Ser-Val-Arg-Gly-Leu Oryctolagus cuniculus ?
-
 ?
2.7.11.19 ATP + synthetic tetradecapeptide i.e. Ser-Asp-Gln-Glu-Lys-Arg-Lys-Gln-Ile-Ser-Val-Arg-Gly-Leu Squalus acanthias ?
-
 ?
2.7.11.19 ATP + synthetic tetradecapeptide phosphorylation site: Ser between Ile and Val Squalus acanthias ?
-
 ?
2.7.11.19 ATP + troponin I not rabbit or dogfish troponin I Oryctolagus cuniculus ADP + phosphotroponin I
-
 ?
2.7.11.19 ATP + troponin I not rabbit or dogfish troponin I Squalus acanthias ADP + phosphotroponin I
-
 ?
2.7.11.19 ATP + troponin T not rabbit or dogfish troponin T Oryctolagus cuniculus ADP + phosphotroponin T
-
 ?
2.7.11.19 ATP + troponin T not rabbit or dogfish troponin T Squalus acanthias ADP + phosphotroponin T
-
 ?
2.7.11.19 GTP + phosphorylase b not (dogfish) Squalus acanthias GDP + phosphorylase a
-
 ?
2.7.11.19 additional information creatine phosphate, phosphoenolpyruvate, actin, parvalbumin, protamin, dogfish or rabbit myosin, adenosine 5'-(3-methyltriphosphate), 5'-adenylylimidodiphosphate (dogfish) are no substrates Oryctolagus cuniculus ?
-
 ?
2.7.11.19 additional information creatine phosphate, phosphoenolpyruvate, actin, parvalbumin, protamin, dogfish or rabbit myosin, adenosine 5'-(3-methyltriphosphate), 5'-adenylylimidodiphosphate (dogfish) are no substrates Squalus acanthias ?
-
 ?
2.7.11.19 additional information ITP, and CTP are no substrates Oryctolagus cuniculus ?
-
 ?
2.7.11.19 additional information ITP, and CTP are no substrates Squalus acanthias ?
-
 ?
2.7.11.19 additional information histone II-A is no substrate Oryctolagus cuniculus ?
-
 ?
2.7.11.19 additional information histone II-A is no substrate Squalus acanthias ?
-
 ?
2.7.11.19 additional information phosvitin is no substrate Oryctolagus cuniculus ?
-
 ?
2.7.11.19 additional information phosvitin is no substrate Squalus acanthias ?
-
 ?
2.7.11.19 UTP + phosphorylase b not (dogfish) Squalus acanthias UDP + phosphorylase a
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Oryctolagus cuniculus
2.7.11.19 30
-
 assay at Squalus acanthias

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 8.5
-
-
 Squalus acanthias

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.19 6.5 8.5 progressive increase of activity, biphasic with dogfish phosphorylase b as substrate: then about half-maximal activity at pH 7.2-7.6 (dogfish activated enzyme) Squalus acanthias
2.7.11.19 6.8 8.5 about half-maximal activity at pH 6.8 and maximal activity at pH 8.5, activated rabbit enzyme Oryctolagus cuniculus
2.7.11.19 8.1 8.5 about half-maximal activity at pH 8.1 and maximal activity at pH 8.5, nonactivated rabbit enzyme Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.7.11.19 6
-
 below, rapid irreversible inactivation Oryctolagus cuniculus
2.7.11.19 6
-
 below, rapid irreversible inactivation Squalus acanthias