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Literature summary extracted from

  • Skuster, J.R.; Chan, K.F.J.; Graves, D.J.
    Isolation and properties of the catalytically active gamma subunit of phosphorylase b kinase (1980), J. Biol. Chem., 255, 2203-2210.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.7.11.19 Incubation of nonactivated enzyme with 100 mM ATP at 0°C dissociates the 23 S enzyme to active 7.5 S and 14 S subunits, with LiBr it produces 5 S subunits Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 EGTA not Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 additional information
-
 additional information kinetic parameters of catalytically active gamma subunit at pH 6.8 and 8.5 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ca2+ stabilization, no absolute requirement for catalytic subunit gamma2 Oryctolagus cuniculus
2.7.11.19 Mg2+ requirement Oryctolagus cuniculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 45000
-
 2 * 45000, rabbit, catalytically active gammagamma subunit, SDS-PAGE Oryctolagus cuniculus
2.7.11.19 86000
-
 catalytically active gammagamma subunit complex, gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 catalytic subunit (i.e. gamma subunit, from holoenzyme by dissociation) Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + nonactivated phosphorylase kinase i.e. autophosphorylation and autoactivation Oryctolagus cuniculus ADP + activated phosphorylase kinase
-
 ?
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + synthetic tetradecapeptide from amino-terminal of phosphorylase b Oryctolagus cuniculus ?
-
 ?
2.7.11.19 additional information no substrates are phosphorylase kinase gamma subunit Oryctolagus cuniculus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.19 dimer 2 * 45000, rabbit, catalytically active gammagamma subunit, SDS-PAGE Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.19 6.2 9.5 about half-maximal activity at pH 6.2 and about 70% of maximal activity at pH 9.5 Oryctolagus cuniculus