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Literature summary extracted from

  • Vandenheede, J.R.; De Wulf, H.; Merlevede, W.
    Liver phosphorylase b kinase. Cyclic-AMP-mediated activation and properties of the partially purified rat-liver enzyme (1979), Eur. J. Biochem., 101, 51-58.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 adenosine 3',5'-monophosphate cAMP mediated activation Rattus norvegicus
2.7.11.19 adenosine 3',5'-monophosphate cAMP mediated activation Oryctolagus cuniculus

General Stability

EC Number General Stability Organism
2.7.11.19 unstable in dilute solutions Rattus norvegicus
2.7.11.19 unstable in dilute solutions Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 EGTA
-
 Oryctolagus cuniculus
2.7.11.19 EGTA
-
 Rattus norvegicus
2.7.11.19 Mg2+ in excess of ATP; nonactivated and activated enzyme Oryctolagus cuniculus
2.7.11.19 Mg2+ in excess of ATP; nonactivated and activated enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 additional information
-
 additional information
-
 Rattus norvegicus
2.7.11.19 additional information
-
 additional information
-
 Oryctolagus cuniculus
2.7.11.19 0.07
-
 MgATP2- activated rabbit skeletal muscle enzyme, pH 8.2 Rattus norvegicus
2.7.11.19 0.07
-
 MgATP2- activated rabbit skeletal muscle enzyme, pH 8.2 Oryctolagus cuniculus
2.7.11.19 0.08
-
 MgATP2- activated rabbit skeletal muscle enzyme, pH 6 Rattus norvegicus
2.7.11.19 0.08
-
 MgATP2- activated rabbit skeletal muscle enzyme, pH 6 Oryctolagus cuniculus
2.7.11.19 0.1
-
 MgATP2- nonactivated rabbit skeletal muscle enzyme, pH 6 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.19 cytosol
-
 Rattus norvegicus 5829
-
2.7.11.19 cytosol
-
 Oryctolagus cuniculus 5829
-
2.7.11.19 soluble
-
 Rattus norvegicus
-
-
2.7.11.19 soluble
-
 Oryctolagus cuniculus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ca2+ required for efficient substrate binding of active and nonactivated enzyme and for maximal catalysis of active enzyme Rattus norvegicus
2.7.11.19 Ca2+ required for efficient substrate binding of active and nonactivated enzyme and for maximal catalysis of active enzyme Oryctolagus cuniculus
2.7.11.19 Mg2+ requirement Rattus norvegicus
2.7.11.19 Mg2+ requirement Oryctolagus cuniculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Rattus norvegicus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Rattus norvegicus
2.7.11.19 phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.19 1300000
-
 rat, gel filtration Rattus norvegicus
2.7.11.19 1300000
-
 rat, gel filtration Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-
2.7.11.19 Rattus norvegicus
-
 male Wistar
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 partial Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 liver
-
 Rattus norvegicus
-
2.7.11.19 liver
-
 Oryctolagus cuniculus
-
2.7.11.19 skeletal muscle
-
 Rattus norvegicus
-
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.19 additional information
-
-
 Rattus norvegicus
2.7.11.19 additional information
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
2.7.11.19 -20°C, in 0.25 M sucrose, 0.1 M Tris-HCl, 0.5 mM DTT, pH 7.4, 50% glycerol, several weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + glycogen synthase inactivation of skeletal muscle glycogen synthase in the presence or absence of EGTA Rattus norvegicus ADP + phosphoglycogen synthase
-
 ?
2.7.11.19 ATP + glycogen synthase inactivation of skeletal muscle glycogen synthase in the presence or absence of EGTA Oryctolagus cuniculus ADP + phosphoglycogen synthase
-
 ?
2.7.11.19 ATP + glycogen synthase at high concentration, from rabbit skeletal muscle Rattus norvegicus ADP + phosphoglycogen synthase
-
 ?
2.7.11.19 ATP + glycogen synthase at high concentration, from rabbit skeletal muscle Oryctolagus cuniculus ADP + phosphoglycogen synthase
-
 ?
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Rattus norvegicus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b rabbit skeletal muscle Rattus norvegicus ADP + phosphorylase a
-
 ?
2.7.11.19 ATP + phosphorylase b rabbit skeletal muscle Oryctolagus cuniculus ADP + phosphorylase a
-
 ?
2.7.11.19 additional information histone H2B is no substrate Rattus norvegicus ?
-
 ?
2.7.11.19 additional information histone H2B is no substrate Oryctolagus cuniculus ?
-
 ?
2.7.11.19 additional information no spontaneous or MnSO4-induced dephosphorylation of activated enzyme Rattus norvegicus ?
-
 ?
2.7.11.19 additional information no spontaneous or MnSO4-induced dephosphorylation of activated enzyme Oryctolagus cuniculus ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Rattus norvegicus
2.7.11.19 30
-
 assay at Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.19 6
-
 nonactivated enzyme Rattus norvegicus
2.7.11.19 7
-
-
 Oryctolagus cuniculus
2.7.11.19 7
-
 above, activated enzyme Rattus norvegicus