Literature summary extracted from
Meyer, W.L.; Fischer, E.H.; Krebs, E.G.
Activation of skeletal muscle phosphorylase b kinase by calcium ion (1964), Biochemistry, 3, 1033-1039.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.11.19 |
Ca2+-dependent protease |
i.e. kinase-activating factor |
Oryctolagus cuniculus |
|
2.7.11.19 |
additional information |
no activation of nonactivated enzyme by renin (with or without Ca2+), thrombin (with or without Ca2+), phospholipase D from Clostridium perfringens or phospholipase from Crotalus adamanteus |
Oryctolagus cuniculus |
|
2.7.11.19 |
additional information |
the nonactivated enzyme is activated either by limited proteolysis |
Oryctolagus cuniculus |
|
2.7.11.19 |
Trypsin |
proteolytic activation of nonactivated enzyme |
Oryctolagus cuniculus |
|
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.7.11.19 |
EDTA |
Ca2+ restores; less effective than EGTA |
Oryctolagus cuniculus |
|
2.7.11.19 |
EGTA |
nonactivated enzyme, more effective than EDTA; strong |
Oryctolagus cuniculus |
|
2.7.11.19 |
additional information |
no inhibition by diethyldithiocarbamic acid, 2,2'-dipyridyl |
Oryctolagus cuniculus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
2.7.11.19 |
0.03 |
0.037 |
ATP |
phosphorylase b |
Oryctolagus cuniculus |
|
2.7.11.19 |
0.03 |
0.037 |
ATP |
activated enzyme, pH 7.5 |
Oryctolagus cuniculus |
|
2.7.11.19 |
0.14 |
- |
phosphorylase b |
before activation, pH 7.5 |
Oryctolagus cuniculus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.7.11.19 |
Ba2+ |
activation, can replace Ca2+ with 60% efficiency |
Oryctolagus cuniculus |
|
2.7.11.19 |
Ca2+ |
irreversible activation of nonactivated kinase by preincubation together with a separate kinase-activating factor independent of cAMP, kinetics |
Oryctolagus cuniculus |
|
2.7.11.19 |
Co2+ |
not |
Oryctolagus cuniculus |
|
2.7.11.19 |
Fe3+ |
not |
Oryctolagus cuniculus |
|
2.7.11.19 |
Mg2+ |
requirement |
Oryctolagus cuniculus |
|
2.7.11.19 |
Mg2+ |
not |
Oryctolagus cuniculus |
|
2.7.11.19 |
Mg2+ |
major role of Mg2+: cosubstrate in Mg2+-ATP complex |
Oryctolagus cuniculus |
|
2.7.11.19 |
Mn2+ |
requirement |
Oryctolagus cuniculus |
|
2.7.11.19 |
Mn2+ |
can substitute for Ca2+ |
Oryctolagus cuniculus |
|
2.7.11.19 |
additional information |
Fe2+, Zn2+ or Ni2+ |
Oryctolagus cuniculus |
|
2.7.11.19 |
phosphate |
requirement, phosphate containing enzyme |
Oryctolagus cuniculus |
|
2.7.11.19 |
Sr2+ |
activation, can replace Ca2+ with 60% efficiency |
Oryctolagus cuniculus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.11.19 |
Oryctolagus cuniculus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.7.11.19 |
partial |
Oryctolagus cuniculus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.7.11.19 |
skeletal muscle |
- |
Oryctolagus cuniculus |
- |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.7.11.19 |
ATP + phosphorylase b |
cosubstrate: Mg-ATP complex |
Oryctolagus cuniculus |
ADP + phosphorylase a |
- |
? |
|
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
2.7.11.19 |
30 |
- |
assay at |
Oryctolagus cuniculus |