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Literature summary extracted from

  • Meyer, W.L.; Fischer, E.H.; Krebs, E.G.
    Activation of skeletal muscle phosphorylase b kinase by calcium ion (1964), Biochemistry, 3, 1033-1039.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.19 Ca2+-dependent protease i.e. kinase-activating factor Oryctolagus cuniculus
2.7.11.19 additional information no activation of nonactivated enzyme by renin (with or without Ca2+), thrombin (with or without Ca2+), phospholipase D from Clostridium perfringens or phospholipase from Crotalus adamanteus Oryctolagus cuniculus
2.7.11.19 additional information the nonactivated enzyme is activated either by limited proteolysis Oryctolagus cuniculus
2.7.11.19 Trypsin proteolytic activation of nonactivated enzyme Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.19 EDTA Ca2+ restores; less effective than EGTA Oryctolagus cuniculus
2.7.11.19 EGTA nonactivated enzyme, more effective than EDTA; strong Oryctolagus cuniculus
2.7.11.19 additional information no inhibition by diethyldithiocarbamic acid, 2,2'-dipyridyl Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.19 0.03 0.037 ATP phosphorylase b Oryctolagus cuniculus
2.7.11.19 0.03 0.037 ATP activated enzyme, pH 7.5 Oryctolagus cuniculus
2.7.11.19 0.14
-
 phosphorylase b before activation, pH 7.5 Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.19 Ba2+ activation, can replace Ca2+ with 60% efficiency Oryctolagus cuniculus
2.7.11.19 Ca2+ irreversible activation of nonactivated kinase by preincubation together with a separate kinase-activating factor independent of cAMP, kinetics Oryctolagus cuniculus
2.7.11.19 Co2+ not Oryctolagus cuniculus
2.7.11.19 Fe3+ not Oryctolagus cuniculus
2.7.11.19 Mg2+ requirement Oryctolagus cuniculus
2.7.11.19 Mg2+ not Oryctolagus cuniculus
2.7.11.19 Mg2+ major role of Mg2+: cosubstrate in Mg2+-ATP complex Oryctolagus cuniculus
2.7.11.19 Mn2+ requirement Oryctolagus cuniculus
2.7.11.19 Mn2+ can substitute for Ca2+ Oryctolagus cuniculus
2.7.11.19 additional information Fe2+, Zn2+ or Ni2+ Oryctolagus cuniculus
2.7.11.19 phosphate requirement, phosphate containing enzyme Oryctolagus cuniculus
2.7.11.19 Sr2+ activation, can replace Ca2+ with 60% efficiency Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.19 Oryctolagus cuniculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.19 partial Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.19 skeletal muscle
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.19 ATP + phosphorylase b cosubstrate: Mg-ATP complex Oryctolagus cuniculus ADP + phosphorylase a
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.19 30
-
 assay at Oryctolagus cuniculus