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Literature summary extracted from

  • Tan, J.L.; Spudich, J.A.
    Dictyostelium myosin light chain kinase. Purification and characterization (1990), J. Biol. Chem., 265, 13818-13824.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.18 autophosphorylation activation Dictyostelium discoideum
2.7.11.18 additional information no activation by cGMP Dictyostelium discoideum
2.7.11.18 additional information no activation by cAMP Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.18 Histone 2A
-
 Dictyostelium discoideum
2.7.11.18 Increasing ionic strength above 0.1 M KCl Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.18 0.004
-
 myosin myosin from Dictyostelium Dictyostelium discoideum
2.7.11.18 0.004
-
 Dictyostelium myosin 22°C, pH 7.5 Dictyostelium discoideum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.18 Ca2+ not Dictyostelium discoideum
2.7.11.18 Ca2+ calcium/calmodulin regulation Dictyostelium discoideum
2.7.11.18 Mg2+
-
 Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.18 34000
-
-
 Dictyostelium discoideum
2.7.11.18 34000
-
 gel filtration Dictyostelium discoideum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.18 ATP + myosin light chain Dictyostelium discoideum the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain ADP + phosphorylated myosin light chain
-
 ?
2.7.11.18 ATP + myosin light chain Dictyostelium discoideum
-
 ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain Dictyostelium discoideum Ax-3 the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain ADP + phosphorylated myosin light chain
-
 ?
2.7.11.18 ATP + myosin light chain Dictyostelium discoideum Ax-3
-
 ADP + myosin light chain phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.18 Dictyostelium discoideum P25323
-
-
2.7.11.18 Dictyostelium discoideum Ax-3 P25323
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.18
-
 Dictyostelium discoideum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.18 0.00615
-
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.18 ATP + Dictyostelium myosin
-
 Dictyostelium discoideum ADP + Dictyostelium myosin phosphate
-
 ?
2.7.11.18 ATP + myosin
-
 Dictyostelium discoideum ADP + myosin phosphate
-
 ?
2.7.11.18 ATP + myosin light chain the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain Dictyostelium discoideum ADP + phosphorylated myosin light chain
-
 ?
2.7.11.18 ATP + myosin light chain the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain Dictyostelium discoideum Ax-3 ADP + phosphorylated myosin light chain
-
 ?
2.7.11.18 ATP + myosin light chain
-
 Dictyostelium discoideum ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrates are myosin heavy chain and phosvitin Dictyostelium discoideum ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is histone 2-A Dictyostelium discoideum ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is casein Dictyostelium discoideum ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain not 16 kDa light chain Dictyostelium discoideum ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrate is phosphorylase b Dictyostelium discoideum ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain
-
 Dictyostelium discoideum Ax-3 ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + myosin light chain no substrates are myosin heavy chain and phosvitin Dictyostelium discoideum Ax-3 ADP + myosin light chain phosphate
-
 ?
2.7.11.18 ATP + protein intramolecular autophosphorylation activates the enzyme Dictyostelium discoideum ADP + phosphoprotein
-
 ?
2.7.11.18 ATP + protein intramolecular autophosphorylation activates the enzyme Dictyostelium discoideum Ax-3 ADP + phosphoprotein
-
 ?
2.7.11.18 additional information skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate Dictyostelium discoideum ?
-
 ?
2.7.11.18 additional information skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate Dictyostelium discoideum Ax-3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.18 monomer 1 * 34000, SDS-PAGE Dictyostelium discoideum

Synonyms

EC Number Synonyms Comment Organism
2.7.11.18 myosin light chain kinase
-
 Dictyostelium discoideum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.18 22
-
 assay at Dictyostelium discoideum

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.18 Calmodulin not Dictyostelium discoideum