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Literature summary extracted from

  • Boulton, T.G.; Gregory, J.S.; Cobb, M.H.
    Purification and properties of extracellular signal-regulated kinase 1, an insulin-stimulated microtubule-associated protein 2 kinase (1991), Biochemistry, 30, 278-286.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.17 2-amino-6-mercaptopurine stimulation Rattus norvegicus
2.7.11.17 isobutylmethylxanthine slight stimulation Rattus norvegicus
2.7.11.17 protamine stimulation Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.17 2-Aminopurine
-
 Rattus norvegicus
2.7.11.17 Ca2+ not Rattus norvegicus
2.7.11.17 Chloroquine
-
 Rattus norvegicus
2.7.11.17 quercetin at high concentrations Rattus norvegicus
2.7.11.17 staurosporine at high concentrations Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Mg2+ activation Rattus norvegicus
2.7.11.17 Mn2+ activation Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.17 43000
-
 x * 43000, SDS-PAGE Rattus norvegicus
2.7.11.24 43000
-
 x * 43000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Rattus norvegicus
-
-
-
2.7.11.24 Rattus norvegicus P21708
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17 insulin stimulated 1HIRc B-cells Rattus norvegicus
2.7.11.24
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.17 fibroblast 1HIRc B cells (overexpressing the human insulin receptor), embryonic cell line 3Y1, both with elevated kinase activity after treatment with mitogens, e.g. insulin or epidermal growth factor Rattus norvegicus
-
2.7.11.24 cell culture insulin-treated rat 1 HIRc B cells Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.17 0.8 1 microtubule associated protein 2 as substrate Rattus norvegicus
2.7.11.17 3
-
 myelin basic protein as substrate Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + histone 5S, 6S, 7S Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + histone 8S Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + histone not: 2AS Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + microtubule-associated protein 2 ATP preferred to GTP Rattus norvegicus ADP + microtubule-associated protein 2 phosphate
-
 ?
2.7.11.17 ATP + microtubule-associated protein 2 phosphorylation sites are serine and threonine Rattus norvegicus ADP + microtubule-associated protein 2 phosphate
-
 ?
2.7.11.17 ATP + myelin basic protein best substrate Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + myelin basic protein phosphorylation sites: threonine Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + myosin light chain poor Rattus norvegicus ?
-
 ?
2.7.11.17 ATP + myosin light chain smooth-muscle, cardiac Rattus norvegicus ?
-
 ?
2.7.11.17 additional information poor or no substrates are kinesin, myosin I, phosvitin, ATP-citrate lyase Rattus norvegicus ?
-
 ?
2.7.11.17 additional information no substrates are protamine Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.17 ? x * 43000, SDS-PAGE Rattus norvegicus
2.7.11.24 ? x * 43000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
2.7.11.24 ERK1
-
 Rattus norvegicus
2.7.11.24 mitogen-activated protein kinase 3
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 30
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.11.17 2.17
-
 myelin basic protein
-
 Rattus norvegicus