EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.75 | diphosphate | - |
Saccharomyces cerevisiae | |
2.5.1.75 | EDTA | - |
Saccharomyces cerevisiae | |
2.5.1.75 | iodoacetamide | - |
Saccharomyces cerevisiae | |
2.5.1.75 | p-mercuribenzoate | - |
Saccharomyces cerevisiae |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.75 | Mg2+ | required | Saccharomyces cerevisiae | |
2.5.1.75 | Mg2+ | optimal concentration: 5 mM | Saccharomyces cerevisiae | |
2.5.1.75 | Mn2+ | can partially replace Mg2+ in activation | Saccharomyces cerevisiae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNA | Saccharomyces cerevisiae | biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.75 | Saccharomyces cerevisiae | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.75 | partial | Saccharomyces cerevisiae |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.75 | additional information | - |
- |
Saccharomyces cerevisiae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNA | specific for DELTA2-isopentenyl phosphate | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNA | no reaction with DELTA3-isopentenyl diphosphate | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNA | no reaction with homologous native tRNA or homologous, permanganate-treated tRNA (permanganate specifically cleaves the DELTA2-isopentenyl groups of tRNA leaving adenosine residues) | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNA | the enzyme from both yeast and rat liver catalyzes a significant incorporation of DELTA2-isopentenyl groups into untreated E. coli B tRNA | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? | |
2.5.1.75 | dimethylallyl diphosphate + adenine37 in tRNA | biosynthesis of N6-(DELTA2-isopentenyl)adenosine which several species of tRNA contain adjacent to the 3'-end of the anticodon | Saccharomyces cerevisiae | diphosphate + N6-dimethylallyladenine37 in tRNA | - |
? |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.75 | 7.5 | 8 | - |
Saccharomyces cerevisiae |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.75 | 7 | 9.5 | pH 7: about 95% of maximum activity, pH 9.5: about 45% of maximum activity | Saccharomyces cerevisiae |