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Literature summary extracted from

  • Cardalda, C.A.; Juknat, A.A.; Princ, F.G.; Batlle, A.
    Rat harderian gland porphobilinogen deaminase: characterization studies and regulatory action of protoporphyrin IX (1997), Arch. Biochem. Biophys., 347, 69-77.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.61 5,5'-dithiobis(2-nitrobenzoic acid) 5 mM, pH 8.0, 50% inhibition Rattus norvegicus
2.5.1.61 additional information
-
 Rattus norvegicus
2.5.1.61 N-ethylmaleimide 5 mM, pH 8.0, 70% inhibition Rattus norvegicus
2.5.1.61 protoporphyrin IX enzyme activity decreases with increasing concentrations, reaching a 33% inhibition at the vivo concentration of porphyrin, 0.00185 mM, and 0.014 mM porphobilinogen Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.61 0.0011
-
 porphobilinogen 37°C, pH 8.0 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.61 38000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.61 porphobilinogen Rattus norvegicus
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.61 Rattus norvegicus
-
 male Chbb Thom rat
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.61
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.61 harderian gland
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.61 additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.61 4 porphobilinogen in the presence of uroporphyrinogen III cosynthase, EC 4.2.1.75 Rattus norvegicus uroporphyrinogen III + 4 NH3
-
 ?
2.5.1.61 4 porphobilinogen + H2O
-
 Rattus norvegicus hydroxylmethylbilane + 4 NH3
-
 ?
2.5.1.61 porphobilinogen
-
 Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.61 PBG-D
-
 Rattus norvegicus
2.5.1.61 porphobilinogen deaminase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.61 60
-
 maximal activity Rattus norvegicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.61 70
-
 10 min, heat-stable up to 70°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.61 8 8.2
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.61 6 10.6 no activity below pH 6.0 and above pH 10.6 Rattus norvegicus