Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kajander, E.O.; Kauppinen, L.I.; Pajula, R.L.; Karkola, K.; Eloranta, T.O.
    Purification and partial characterization of human polyamine synthases (1989), Biochem. J., 259, 879-886.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 5'-methylthioadenosine
-
 Bos taurus
2.5.1.16 5'-methylthioadenosine
-
 Homo sapiens
2.5.1.16 5'-methylthioadenosine
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.007
-
 S-adenosyl-3-methylthio-1-propylamine pH 7.4, 37ºC, spleen Homo sapiens
2.5.1.16 0.08
-
 1,4-diaminobutane pH 7.4, 37ºC, spleen Homo sapiens
2.5.1.22 0.001
-
 S-adenosylmethioninamine
-
 Homo sapiens
2.5.1.22 0.2
-
 spermidine placenta Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.16 cytosol
-
 Homo sapiens 5829
-
2.5.1.16 cytosol
-
 Bos taurus 5829
-
2.5.1.22 cytosol
-
 Homo sapiens 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 35000
-
 2 * 35000, SDS-PAGE Homo sapiens
2.5.1.16 62000
-
 spleen, pore gradient gel electrophoresis Homo sapiens
2.5.1.16 62000
-
 brain, pore gradient gel electrophoresis Bos taurus
2.5.1.22 45000
-
 2 * 45000, kidney, SDS-PAGE Homo sapiens
2.5.1.22 78000
-
 kidney, pore-gradient electrophoresis Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Bos taurus
-
-
-
2.5.1.16 Homo sapiens
-
-
-
2.5.1.16 Rattus norvegicus
-
-
-
2.5.1.22 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16
-
 Rattus norvegicus
2.5.1.16 affinity chromatography Bos taurus
2.5.1.16 ammonium sulfate fractionation, chromatography on DEAE-Cellulose, affinity chromatography, 8700fold purified Homo sapiens
2.5.1.22
-
 Homo sapiens

Reaction

EC Number Reaction Comment Organism Reaction ID
2.5.1.16 S-adenosyl 3-(methylsulfanyl)propylamine + putrescine = S-methyl-5'-thioadenosine + spermidine Michaelis-Menten kinetics Homo sapiens
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.16 brain
-
 Homo sapiens
-
2.5.1.16 brain
-
 Rattus norvegicus
-
2.5.1.16 brain
-
 Bos taurus
-
2.5.1.16 kidney
-
 Homo sapiens
-
2.5.1.16 kidney
-
 Rattus norvegicus
-
2.5.1.16 lung
-
 Homo sapiens
-
2.5.1.16 additional information tissue distribution Homo sapiens
-
2.5.1.16 additional information tissue distribution Rattus norvegicus
-
2.5.1.16 pancreas
-
 Homo sapiens
-
2.5.1.16 pancreas
-
 Rattus norvegicus
-
2.5.1.16 placenta
-
 Homo sapiens
-
2.5.1.16 spleen
-
 Homo sapiens
-
2.5.1.22 brain
-
 Homo sapiens
-
2.5.1.22 kidney
-
 Homo sapiens
-
2.5.1.22 additional information tissue distribution Homo sapiens
-
2.5.1.22 placenta
-
 Homo sapiens
-
2.5.1.22 spleen
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.16 0.66
-
-
 Homo sapiens
2.5.1.22 0.455
-
 placenta Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.5.1.16 -80ºC, 20% glycerol, rapid loss of activity in pancreas preparations Homo sapiens
2.5.1.16 -80ºC, 20% glycerol, stable for spleen tissue enzyme during storage and freeze-thawings Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.22 S-adenosyl-L-methioninamine + spermidine
-
 Homo sapiens 5'-methylthioadenosine + spermine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer
-
 Rattus norvegicus
2.5.1.16 dimer
-
 Bos taurus
2.5.1.16 dimer 2 * 35000, SDS-PAGE Homo sapiens
2.5.1.22 dimer 2 * 45000, kidney, SDS-PAGE Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.16 37
-
 assay at Homo sapiens
2.5.1.16 37
-
 assay at Rattus norvegicus
2.5.1.16 37
-
 assay at Bos taurus
2.5.1.22 37
-
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.16 additional information
-
-
 Rattus norvegicus
2.5.1.16 additional information
-
-
 Bos taurus
2.5.1.16 additional information
-
 pI 5.1, in spleen Homo sapiens