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Literature summary extracted from

  • Fyfe, J.A.; Friedmann, H.C.
    Vitamin B 12 biosynthesis. Enzyme studies on the formation of the alpha-glycosidic nucleotide precursor (1969), J. Biol. Chem., 244, 1659-1666.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.21 0.01
-
 adenine
-
 Acetoanaerobium sticklandii
2.4.2.21 0.3
-
 nicotinic acid mononucleotide cosubstrate adenine Acetoanaerobium sticklandii
2.4.2.21 0.5
-
 benzimidazole
-
 Acetoanaerobium sticklandii
2.4.2.21 0.7
-
 nicotinic acid mononucleotide cosubstrate benzimidazole Acetoanaerobium sticklandii

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.21 Acetoanaerobium sticklandii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.21
-
 Acetoanaerobium sticklandii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.21 beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole = nicotinate + alpha-ribazole 5'-phosphate + H+ single displacement mechanism Acetoanaerobium sticklandii
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.21 2.3
-
-
 Acetoanaerobium sticklandii

Storage Stability

EC Number Storage Stability Organism
2.4.2.21 -15°C, 0.01 M potassium phosphate buffer, pH 6.8, 0.005 M DTT, 10% ethylene glycol, several months Acetoanaerobium sticklandii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.21 beta-nicotinate D-ribonucleotide + 5(6)-nitrobenzimidazole
-
 Acetoanaerobium sticklandii nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5(6)-nitrobenzimidazole
-
 ?
2.4.2.21 beta-nicotinate D-ribonucleotide + 5,6-dichlorobenzimidazole
-
 Acetoanaerobium sticklandii nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5,6-dichlorobenzimidazole
-
 ?
2.4.2.21 beta-nicotinate D-ribonucleotide + 5,6-dimethylbenzimidazole
-
 Acetoanaerobium sticklandii nicotinate + N1-(5-phospho-alpha-D-ribosyl)-5,6-dimethylbenzimidazole
-
 ?
2.4.2.21 nicotinic acid mononucleotide + adenine
-
 Acetoanaerobium sticklandii 7-alpha-D-ribofuranosyladenine 5'-phosphate + nicotinate
-
 ?
2.4.2.21 nicotinic acid mononucleotide + benzimidazole highly specific for nicotinic acid mononucleotide Acetoanaerobium sticklandii 1-alpha-D-ribofuranosylbenzimidazole 5'-phosphate + nicotinate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.21 37
-
 assay at Acetoanaerobium sticklandii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.21 8.6
-
 assay at Acetoanaerobium sticklandii
2.4.2.21 8.6 9.2 nicotinic acid mononucleotide + adenine or benzimidazole Acetoanaerobium sticklandii