BRENDA - Enzyme Database

Human glutamine phosphoribosylpyrophosphate amidotransferase. Kinetic and regulatory properties

Holmes, E.W.; McDonald, J.A.; McCord, J.M.; Wyngaarden, J.B.; Kelley, W.N.; J. Biol. Chem. 248, 144-150 (1973)

Data extracted from this reference:

General Stability
EC Number
General Stability
Organism
2.4.2.14
Mg2+ and phosphate are essential for stability
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.14
6-iodopurine
5 mM, 39% inhibition
Homo sapiens
2.4.2.14
6-mercaptopurine ribonucleotide
5 mM, 64% inhibition
Homo sapiens
2.4.2.14
ADP
5 mM, 40% inhibition
Homo sapiens
2.4.2.14
allopurinol ribonucleotide
5 mM, 68% inhibition
Homo sapiens
2.4.2.14
AMP
5 mM, 76% inhibition, noncompetive vs. glutamine
Homo sapiens
2.4.2.14
ATP
5 mM, 21% inhibition
Homo sapiens
2.4.2.14
CMP
5 mM, 22% inhibition
Homo sapiens
2.4.2.14
dCMP
5 mM, 26% inhibition
Homo sapiens
2.4.2.14
GDP
5 mM, 40% inhibition
Homo sapiens
2.4.2.14
GMP
0.5 mM; 5 mM, 70% inhibition
Homo sapiens
2.4.2.14
GTP
5 mM, 13% inhibition
Homo sapiens
2.4.2.14
IDP
5 mM, 29% inhibition
Homo sapiens
2.4.2.14
IMP
5 mM, 41% inhibition
Homo sapiens
2.4.2.14
methyl-dCMP
5 mM, 47% inhibition
Homo sapiens
2.4.2.14
OMP
5 mM, 25% inhibition
Homo sapiens
2.4.2.14
phosphate
competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
2.4.2.14
TMP
5 mM, 42% inhibition
Homo sapiens
2.4.2.14
TTP
5 mM, 12% inhibition
Homo sapiens
2.4.2.14
UDP
5 mM, 19% inhibition
Homo sapiens
2.4.2.14
UMP
5 mM, 25% inhibition
Homo sapiens
2.4.2.14
UTP
5 mM, 16% inhibition
Homo sapiens
2.4.2.14
XDP
5 mM, 27% inhibition
Homo sapiens
2.4.2.14
XMP
5 mM, 41% inhibition
Homo sapiens
2.4.2.14
XTP
5 mM, 44% inhibition
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.14
additional information
-
additional information
effect of AMP on kinetic parameters
Homo sapiens
2.4.2.14
0.48
-
5-phospho-alpha-D-ribose 1-diphosphate
in Tris buffer
Homo sapiens
2.4.2.14
0.65
-
Mg2+
-
Homo sapiens
2.4.2.14
1
4.5
L-glutamine
-
Homo sapiens
2.4.2.14
1.6
-
L-glutamine
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.14
cytosol
little or no activity in mitochondria or microsomes
Homo sapiens
5829
-
2.4.2.14
soluble
-
Homo sapiens
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.14
Ca2+
activation, approx. 15% as effective as Mg2+ or Mn2+
Homo sapiens
2.4.2.14
Co2+
activation, approx. 40% as effective as Mg2+ or Mn2+
Homo sapiens
2.4.2.14
Mg2+
Km-value: 0.65 mM, inhibition above 10 mM; required
Homo sapiens
2.4.2.14
Mn2+
required, equally effective as Mg2+
Homo sapiens
2.4.2.14
additional information
not activated by Ba2+, Cd2+, Cu2+, Fe2+, Hg2+, Ni2+, Zn2+
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.14
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
Homo sapiens
regulating enzyme of purine biosynthesis
L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
Homo sapiens
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.2.14
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.4.2.14
partial
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.14
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.2.14
0.0055
-
-
Homo sapiens
Storage Stability
EC Number
Storage Stability
Organism
2.4.2.14
4░C, 50 mM phosphate bufer, pH 7.4, 5 mM MgCl2, 60 mM 2-mercaptoethanol, at least 4 weeks, no loss of activity
Homo sapiens
2.4.2.14
4░C, partially purified preparation in 50 mM phosphate buffer, pH 7.4, 5 mM Mg2+ and 60 mM 2-mercaptoethanol, at least 4 weeks
Homo sapiens
2.4.2.14
4░C, phosphate buffer, 10 d, 40% loss of activity
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.14
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
489757
Homo sapiens
5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
489757
Homo sapiens
-
2.4.2.14
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
regulating enzyme of purine biosynthesis
489757
Homo sapiens
L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
489757
Homo sapiens
?
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.14
6
8
broad, phosphate buffer
Homo sapiens
2.4.2.14
6.5
-
imidazole/HCl buffer
Homo sapiens
2.4.2.14
7.5
-
Tris/HCl buffer
Homo sapiens
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.2.14
Mg2+ and phosphate are essential for stability
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.2.14
6-iodopurine
5 mM, 39% inhibition
Homo sapiens
2.4.2.14
6-mercaptopurine ribonucleotide
5 mM, 64% inhibition
Homo sapiens
2.4.2.14
ADP
5 mM, 40% inhibition
Homo sapiens
2.4.2.14
allopurinol ribonucleotide
5 mM, 68% inhibition
Homo sapiens
2.4.2.14
AMP
5 mM, 76% inhibition, noncompetive vs. glutamine
Homo sapiens
2.4.2.14
ATP
5 mM, 21% inhibition
Homo sapiens
2.4.2.14
CMP
5 mM, 22% inhibition
Homo sapiens
2.4.2.14
dCMP
5 mM, 26% inhibition
Homo sapiens
2.4.2.14
GDP
5 mM, 40% inhibition
Homo sapiens
2.4.2.14
GMP
0.5 mM; 5 mM, 70% inhibition
Homo sapiens
2.4.2.14
GTP
5 mM, 13% inhibition
Homo sapiens
2.4.2.14
IDP
5 mM, 29% inhibition
Homo sapiens
2.4.2.14
IMP
5 mM, 41% inhibition
Homo sapiens
2.4.2.14
methyl-dCMP
5 mM, 47% inhibition
Homo sapiens
2.4.2.14
OMP
5 mM, 25% inhibition
Homo sapiens
2.4.2.14
phosphate
competitive vs. 5-phospho-alpha-D-ribose 1-diphosphate
Homo sapiens
2.4.2.14
TMP
5 mM, 42% inhibition
Homo sapiens
2.4.2.14
TTP
5 mM, 12% inhibition
Homo sapiens
2.4.2.14
UDP
5 mM, 19% inhibition
Homo sapiens
2.4.2.14
UMP
5 mM, 25% inhibition
Homo sapiens
2.4.2.14
UTP
5 mM, 16% inhibition
Homo sapiens
2.4.2.14
XDP
5 mM, 27% inhibition
Homo sapiens
2.4.2.14
XMP
5 mM, 41% inhibition
Homo sapiens
2.4.2.14
XTP
5 mM, 44% inhibition
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.2.14
additional information
-
additional information
effect of AMP on kinetic parameters
Homo sapiens
2.4.2.14
0.48
-
5-phospho-alpha-D-ribose 1-diphosphate
in Tris buffer
Homo sapiens
2.4.2.14
0.65
-
Mg2+
-
Homo sapiens
2.4.2.14
1
4.5
L-glutamine
-
Homo sapiens
2.4.2.14
1.6
-
L-glutamine
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.2.14
cytosol
little or no activity in mitochondria or microsomes
Homo sapiens
5829
-
2.4.2.14
soluble
-
Homo sapiens
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.2.14
Ca2+
activation, approx. 15% as effective as Mg2+ or Mn2+
Homo sapiens
2.4.2.14
Co2+
activation, approx. 40% as effective as Mg2+ or Mn2+
Homo sapiens
2.4.2.14
Mg2+
Km-value: 0.65 mM, inhibition above 10 mM; required
Homo sapiens
2.4.2.14
Mn2+
required, equally effective as Mg2+
Homo sapiens
2.4.2.14
additional information
not activated by Ba2+, Cd2+, Cu2+, Fe2+, Hg2+, Ni2+, Zn2+
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.2.14
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
Homo sapiens
regulating enzyme of purine biosynthesis
L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
Homo sapiens
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.2.14
partial
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.2.14
placenta
-
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.2.14
0.0055
-
-
Homo sapiens
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.2.14
4░C, 50 mM phosphate bufer, pH 7.4, 5 mM MgCl2, 60 mM 2-mercaptoethanol, at least 4 weeks, no loss of activity
Homo sapiens
2.4.2.14
4░C, partially purified preparation in 50 mM phosphate buffer, pH 7.4, 5 mM Mg2+ and 60 mM 2-mercaptoethanol, at least 4 weeks
Homo sapiens
2.4.2.14
4░C, phosphate buffer, 10 d, 40% loss of activity
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.2.14
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
-
489757
Homo sapiens
5-phospho-beta-D-ribosylamine + L-glutamate + diphosphate
-
489757
Homo sapiens
-
2.4.2.14
L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O
regulating enzyme of purine biosynthesis
489757
Homo sapiens
L-glutamate + 5-phospho-beta-D-ribosylamine + diphosphate
-
489757
Homo sapiens
?
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.2.14
6
8
broad, phosphate buffer
Homo sapiens
2.4.2.14
6.5
-
imidazole/HCl buffer
Homo sapiens
2.4.2.14
7.5
-
Tris/HCl buffer
Homo sapiens