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Literature summary extracted from

  • Jones, M.E.; Kavipurapu, P.R.; Traut, T.W.
    Orotate phosphoribosyltransferase: orotidylate decarboxylase (Ehrlich ascites cell) (1978), Methods Enzymol., 51, 155-167.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.10 5 mM Mg2+, 1 mM phosphoribose diphosphate and 2 mM DTT stabilize dilute enzyme solutions Mus musculus
2.4.2.10 dialysis against phosphate buffer, pH 7 or 7.5, unstable to, 2 mM DTT protect Mus musculus
2.4.2.10 dilution inactivates Mus musculus
2.4.2.10 phosphate, 0.3 M and above, stabilizes Mus musculus
2.4.2.10 proteins, e.g. albumin, do not stabilize dilute enzyme solutions Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.10 5-Bromouracil
-
Mus musculus
2.4.2.10 5-Chlorouracil
-
Mus musculus
2.4.2.10 5-Fluoroorotate at 0.05 M Mus musculus
2.4.2.10 adenine
-
Mus musculus
2.4.2.10 adenosine
-
Mus musculus
2.4.2.10 ADP
-
Mus musculus
2.4.2.10 allopurinol
-
Mus musculus
2.4.2.10 AMP
-
Mus musculus
2.4.2.10 ATP
-
Mus musculus
2.4.2.10 Azauracil
-
Mus musculus
2.4.2.10 Azauridine
-
Mus musculus
2.4.2.10 Barbituric acid
-
Mus musculus
2.4.2.10 cytosine
-
Mus musculus
2.4.2.10 dihydroorotate 10 mM Mus musculus
2.4.2.10 diphosphate
-
Mus musculus
2.4.2.10 dUMP
-
Mus musculus
2.4.2.10 GMP
-
Mus musculus
2.4.2.10 IMP
-
Mus musculus
2.4.2.10 additional information not inhibitory: CMP, TMP, 6-aza-UMP or 5-bromo-UMP Mus musculus
2.4.2.10 Orotidine 10 mM Mus musculus
2.4.2.10 Oxipurinol
-
Mus musculus
2.4.2.10 phosphate
-
Mus musculus
2.4.2.10 ribose 5-phosphate not inhibitory Mus musculus
2.4.2.10 UDP
-
Mus musculus
2.4.2.10 UMP 1 mM, not 0.1 mM Mus musculus
2.4.2.10 Uracil
-
Mus musculus
2.4.2.10 uridine
-
Mus musculus
2.4.2.10 UTP
-
Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.10 additional information
-
additional information principles of assay Mus musculus
2.4.2.10 0.002
-
Orotate
-
Mus musculus
2.4.2.10 0.016
-
5-phospho-alpha-D-ribose 1-diphosphate
-
Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.10 Mg2+ requirement Mus musculus
2.4.2.10 Mg2+ Km-value: 3 mM Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.10 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.10
-
Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.10 Ehrlich ascites carcinoma cell
-
Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.10 0.115
-
-
Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.4.2.10 -60°C to-20°C, crude enzyme or ammonium sulfate preparation, 2 years Mus musculus
2.4.2.10 frozen, partially purified enzyme in phosphate buffer, pH 7 or 7.5, 2 months Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.10 orotate + 5-phospho-alpha-D-ribose 1-diphosphate
-
Mus musculus orotidine 5'-phosphate + diphosphate i.e. orotidylate or OMP ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.10 More constitutes together with orotidylate decarboxylase (EC 4.1.1.23) UMP-synthase, former complex U or multienzyme pyr-5,6 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.10 37
-
assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.10 7 7.5 preferred buffer: Tris-HCl, when substrates are non-saturating, phosphate buffer, when substrate are saturating, not: maleate, imidazole, N-tris[hydroxymethyl] methyl-2-aminoethane-sulfonic acid (i.e. TES) or 3-[N-morpholino]-2-hydroxypropanesulfonic acid (i.e. MOPS) buffer Mus musculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.2.10 4 9 stable Mus musculus