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Literature summary extracted from

  • Shirae, H.; Yokozeki, K.
    Purification and properties of purine nucleoside phosphorylase from Brevibacterium acetylicum ATCC 954 (1991), Agric. Biol. Chem., 55, 493-499.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 AgNO3
-
 Exiguobacterium acetylicum
2.4.2.1 CuSO4 slight Exiguobacterium acetylicum
2.4.2.1 HgCl2
-
 Exiguobacterium acetylicum
2.4.2.1 NEM
-
 Exiguobacterium acetylicum
2.4.2.1 PCMB
-
 Exiguobacterium acetylicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 31000
-
 1 * 31000, SDS-PAGE in absence and presence of 2-mercaptoethanol Exiguobacterium acetylicum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Exiguobacterium acetylicum
-
 ATCC 954
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Exiguobacterium acetylicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 195.1
-
-
 Exiguobacterium acetylicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2',3'-dideoxyinosine + phosphate 22% of the activity with inosine Exiguobacterium acetylicum alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
-
 ?
2.4.2.1 deoxyguanosine + phosphate 11% of the activity with inosine Exiguobacterium acetylicum guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate 2'-deoxyinosine, 87% of the activity with inosine Exiguobacterium acetylicum hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate 21% of the activity with inosine Exiguobacterium acetylicum guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate reaction reaches an equilibrium when 21% of the inosine is phosphorolyzed Exiguobacterium acetylicum hypoxanthine + alpha-D-ribose 1-phosphate
-
 r
2.4.2.1 ribavirin + phosphate 22% of the activity with inosine. Phosphorolysis reaches an equilibrium when 15% of ribavirin is phosphorolyzed Exiguobacterium acetylicum alpha-D-ribose 1-phosphate + 1,2,4-triazole-3-carboxamide
-
 r
2.4.2.1 xanthosine + phosphate 26% of the activity with inosine Exiguobacterium acetylicum alpha-D-ribose 1-phosphate + xanthine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 monomer 1 * 31000, SDS-PAGE in absence and presence of 2-mercaptoethanol Exiguobacterium acetylicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 70
-
 phosphorylation of inosine Exiguobacterium acetylicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.2.1 30 80 30°C: about 35% of maximal activity, 80°C: about 85% of maximal activity Exiguobacterium acetylicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 8.5
-
 phosphorylation of inosine Exiguobacterium acetylicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.1 6 9.5 pH 6.0: about 60% of maximal activity, pH 9.5: about 80% of maximal activity Exiguobacterium acetylicum