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Literature summary extracted from

  • Shirae, H.; Yokozeki, K.
    Purifications and properties of orotidine-phosphorolyzing enzyme and purine nucleoside phosphorylase from Erwinia carotovora AJ 2992 (1991), Agric. Biol. Chem., 55, 1849-1857.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 AgNO3
-
Pectobacterium carotovorum
2.4.2.1 CuSO4
-
Pectobacterium carotovorum
2.4.2.1 HgCl2
-
Pectobacterium carotovorum
2.4.2.1 PCMB
-
Pectobacterium carotovorum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 1.85
-
guanosine at 40°C Pectobacterium carotovorum
2.4.2.1 1.92
-
Inosine at 40°C Pectobacterium carotovorum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 58000
-
non-denaturing PAGE Pectobacterium carotovorum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Pectobacterium carotovorum
-
AJ 2992
-
2.4.2.1 Pectobacterium carotovorum AJ 2992
-
AJ 2992
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
Pectobacterium carotovorum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 48.5
-
-
Pectobacterium carotovorum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 2',3'-dideoxyinosine + phosphate 12% of the activity with inosine Pectobacterium carotovorum alpha-D-2,3-dideoxyribose 1-phosphate + hypoxanthine
-
?
2.4.2.1 adenosine + phosphate 48% of the activity with inosine Pectobacterium carotovorum adenine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 adenosine + phosphate 48% of the activity with inosine Pectobacterium carotovorum AJ 2992 adenine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 deoxyadenosine + phosphate 2'-deoxyadenosine, 57% of the activity with inosine. 3'-deoxyadenosine, 7% of the activity with inosine Pectobacterium carotovorum alpha-D-ribose 1-phosphate + adenine
-
?
2.4.2.1 deoxyguanosine + phosphate 2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine Pectobacterium carotovorum guanine + alpha-D-deoxyribose 1-phosphate
-
?
2.4.2.1 deoxyguanosine + phosphate 2'-deoxyguanosine, 41% of the activity with inosine. 3'-deoxyguanosine, 16% of the activity with inosine Pectobacterium carotovorum AJ 2992 guanine + alpha-D-deoxyribose 1-phosphate
-
?
2.4.2.1 deoxyinosine + phosphate 136% of the activity with inosine Pectobacterium carotovorum hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
2.4.2.1 deoxyinosine + phosphate 136% of the activity with inosine Pectobacterium carotovorum AJ 2992 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
r
2.4.2.1 guanosine + phosphate 2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine Pectobacterium carotovorum guanine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 guanosine + phosphate 2'-deoxyinosine, 27% of the activity with inosine. 3'-deoxyinosine, 10% of the activity with inosine Pectobacterium carotovorum AJ 2992 guanine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 inosine + phosphate
-
Pectobacterium carotovorum hypoxanthine + alpha-D-ribose 1-phosphate
-
?
2.4.2.1 inosine + phosphate
-
Pectobacterium carotovorum AJ 2992 hypoxanthine + alpha-D-ribose 1-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 dimer 2 * 58000, SDS-PAGE Pectobacterium carotovorum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 40
-
-
Pectobacterium carotovorum
2.4.2.1 70
-
-
Pectobacterium carotovorum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 8.5
-
-
Pectobacterium carotovorum