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Literature summary extracted from

  • Hori, N.; Watanabe, M.; Yamazaki, Y.; Mikami, Y.
    Purification and characterization of thermostable purine nucleoside phosphorylase of Bacillus stearothermophilus JTS 859 (1989), Agric. Biol. Chem., 53, 2205-2210.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.1 HgCl2
-
 Geobacillus stearothermophilus
2.4.2.1 PCMB
-
 Geobacillus stearothermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.1 0.1
-
 2'-deoxyguanosine
-
 Geobacillus stearothermophilus
2.4.2.1 0.14
-
 guanosine
-
 Geobacillus stearothermophilus
2.4.2.1 0.2
-
 deoxyinosine
-
 Geobacillus stearothermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.1 34000
-
 2 * 34000, SDS-PAGE Geobacillus stearothermophilus
2.4.2.1 68000
-
 gel filtration Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.1 Geobacillus stearothermophilus
-
 JTS 859
-
2.4.2.1 Geobacillus stearothermophilus JTS 859
-
 JTS 859
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.1
-
 Geobacillus stearothermophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.1 405
-
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.1 deoxyguanosine + phosphate
-
 Geobacillus stearothermophilus guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyguanosine + phosphate
-
 Geobacillus stearothermophilus JTS 859 guanine + alpha-D-deoxyribose 1-phosphate
-
 ?
2.4.2.1 deoxyinosine + phosphate
-
 Geobacillus stearothermophilus hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 deoxyinosine + phosphate
-
 Geobacillus stearothermophilus JTS 859 hypoxanthine + alpha-D-deoxyribose 1-phosphate
-
 r
2.4.2.1 guanosine + phosphate
-
 Geobacillus stearothermophilus guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 guanosine + phosphate
-
 Geobacillus stearothermophilus JTS 859 guanine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Geobacillus stearothermophilus hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.1 inosine + phosphate
-
 Geobacillus stearothermophilus JTS 859 hypoxanthine + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.1 dimer 2 * 34000, SDS-PAGE Geobacillus stearothermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.1 80
-
-
 Geobacillus stearothermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.1 70
-
 30 h, stable Geobacillus stearothermophilus
2.4.2.1 80
-
 half-life is 16 h in 20 mM potassium phosphate and 1 mM inosine, pH 7.0 Geobacillus stearothermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.1 7.5 11
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.1 6 12 pH 6.0: about 65% of maximal activity, pH 12.0: about 25% of maximal activity Geobacillus stearothermophilus