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Literature summary extracted from

  • Khalkhali, Z.; Marshall, R.D.; Reuvers, F.; Habets-Willems, C.; Boer, P.
    Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes (1976), Biochem. J., 160, 37-41.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.94 tunicamycin
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.94 membrane
-
 Saccharomyces cerevisiae 16020
-
2.4.1.94 membrane
-
 Oryctolagus cuniculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.94 Mn2+
-
 Oryctolagus cuniculus
2.4.1.94 Mn2+ 2fold activation at 25 mM, slight activation at 20 mM and above 50 mM Saccharomyces cerevisiae

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.94 Oryctolagus cuniculus
-
-
-
2.4.1.94 Saccharomyces cerevisiae
-
 mutant 2180-1A-5mnn2
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.1.94 UDP-N-acetyl-D-glucosamine + [protein]-L-asparagine = UDP + [protein]-N4-(N-acetyl-D-glucosaminyl)-L-asparagine sequence Asn-X-Thr(Ser) at glycosylation site Asn-34 is necessary Saccharomyces cerevisiae
a
2.4.1.94 UDP-N-acetyl-D-glucosamine + [protein]-L-asparagine = UDP + [protein]-N4-(N-acetyl-D-glucosaminyl)-L-asparagine sequence Asn-X-Thr(Ser) at glycosylation site Asn-34 is necessary Oryctolagus cuniculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.94 liver
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.94 UDP-N-acetyl-D-glucosamine + protein
-
 Saccharomyces cerevisiae UDP + 4-N-(N-acetyl-D-glucosaminyl)-protein
-
 ?
2.4.1.94 UDP-N-acetyl-D-glucosamine + protein
-
 Oryctolagus cuniculus UDP + 4-N-(N-acetyl-D-glucosaminyl)-protein
-
 ?
2.4.1.94 UDP-N-acetylglucosamine + pancreatic ribonuclease A
-
 Saccharomyces cerevisiae UDP + pancreatic ribonuclease A with N-acetylglucosamine linked to Asp-34
-
 ?
2.4.1.94 UDP-N-acetylglucosamine + pancreatic ribonuclease A
-
 Oryctolagus cuniculus UDP + pancreatic ribonuclease A with N-acetylglucosamine linked to Asp-34
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.94 37
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.94 7.2
-
 assay at Saccharomyces cerevisiae