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Literature summary extracted from

  • Hrazdina, G.
    Purification and properies of a UDPglucose: flavonoid 3-O-glucosyltransferase from Hippeastrum petals (1988), Biochim. Biophys. Acta, 955, 301-309.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.91 0.0125
-
 kaempferol
-
 Hippeastrum sp.
2.4.1.91 0.07
-
 quercetin about Hippeastrum sp.
2.4.1.91 0.07
-
 cyanidin about Hippeastrum sp.
2.4.1.91 1
-
 UDPglucose
-
 Hippeastrum sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.91 24500
-
 2 * 24500, SDS-PAGE Hippeastrum sp.
2.4.1.91 49000
-
 gel filtration Hippeastrum sp.

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.91 Hippeastrum sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.91
-
 Hippeastrum sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.91 petal
-
 Hippeastrum sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.91 additional information
-
-
 Hippeastrum sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.91 UDP-glucose + isosakuranetin at 11.2% the activity with kaempferol Hippeastrum sp. UDP + ?
-
 ?
2.4.1.91 UDP-glucose + pelargonidin at 4% the activity with kaempferol Hippeastrum sp. UDP + pelargonidin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + apigenin at 4.2% the activity with kaempferol Hippeastrum sp. UDP + apigenin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + cyanidin at 5% the activity with kaempferol Hippeastrum sp. UDP + cyanidin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + dihydrokaempferol 9.6% of the activity with kaempferol Hippeastrum sp. UDP + dihydrokaempferol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + dihydroquercetin
-
 Hippeastrum sp. UDP + dihydroquercetin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + kaempferol
-
 Hippeastrum sp. UDP + kaempferol 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + malvidin at 4% the activity with kaempferol Hippeastrum sp. UDP + malvidin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + naringenin at 3.2% the activity with kaempferol Hippeastrum sp. UDP + naringenin 3-O-glucoside
-
 ?
2.4.1.91 UDPglucose + quercetin 61.2% of the activity with kaempferol Hippeastrum sp. UDP + quercetin 3-O-glucoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.91 dimer 2 * 24500, SDS-PAGE Hippeastrum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.91 5
-
-
 Hippeastrum sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.91 4 9 pH 4.0: about 60% of maximal activity, pH 9.0: about 70% of maximal activity Hippeastrum sp.