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Literature summary extracted from

  • Park, J.E.; Lee, K.Y.; Do, S.I.; Lee, S.S.
    Expression and characterization of beta-1,4-galactosyltransferase from Neisseria meningitidis and Neisseria gonorrhoeae (2002), J. Biochem. Mol. Biol., 35, 330-336.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.38 Triton X-100 0.1-5%, 1.5fold stimulation Neisseria gonorrhoeae
2.4.1.38 Triton X-100 0.1-5%, 1.5fold stimulation Neisseria meningitidis
2.4.1.90 Triton X-100 0.1-5%, 1.5fold stimulation Neisseria gonorrhoeae
2.4.1.90 Triton X-100 0.1-5%, 1.5fold stimulation Neisseria meningitidis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.38 expression under the control of the T7 promoter in Escherichia coli BL21 Neisseria gonorrhoeae
2.4.1.38 expression under the control of the T7 promoter in Escherichia coli BL21 Neisseria meningitidis
2.4.1.90 expression under the control of the T7 promoter in Escherichia coli BL21 Neisseria gonorrhoeae
2.4.1.90 expression under the control of the T7 promoter in Escherichia coli BL21 Neisseria meningitidis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.38 Cu2+ complete inhibition in presence of Mn2+ Neisseria gonorrhoeae
2.4.1.38 Cu2+ complete inhibition in presence of Mn2+ Neisseria meningitidis
2.4.1.38 Zn2+ complete inhibition in presence of Mn2+ Neisseria gonorrhoeae
2.4.1.38 Zn2+ complete inhibition in presence of Mn2+ Neisseria meningitidis
2.4.1.90 Cu2+ complete inhibition in presence of Mn2+ Neisseria gonorrhoeae
2.4.1.90 Cu2+ complete inhibition in presence of Mn2+ Neisseria meningitidis
2.4.1.90 Zn2+ complete inhibition in presence of Mn2+ Neisseria gonorrhoeae
2.4.1.90 Zn2+ complete inhibition in presence of Mn2+ Neisseria meningitidis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.38 Ca2+ about 50% of the activity with Mn2+ Neisseria gonorrhoeae
2.4.1.38 Ca2+ about 50% of the activity with Mn2+ Neisseria meningitidis
2.4.1.38 Fe2+ no activation Neisseria gonorrhoeae
2.4.1.38 Fe2+ partial activation Neisseria meningitidis
2.4.1.38 Mg2+ about 50% of the activity with Mn2+ Neisseria gonorrhoeae
2.4.1.38 Mg2+ about 50% of the activity with Mn2+ Neisseria meningitidis
2.4.1.38 Mn2+ required Neisseria gonorrhoeae
2.4.1.38 Mn2+ required Neisseria meningitidis
2.4.1.38 Mn2+ no activation in presence of Fe2+, Zn2+ and Cu2+ Neisseria gonorrhoeae
2.4.1.38 Mn2+ no activation in presence of Fe2+, Zn2+ and Cu2+ Neisseria meningitidis
2.4.1.90 Ca2+ about 50% of the activity with Mn2+ Neisseria gonorrhoeae
2.4.1.90 Ca2+ about 50% of the activity with Mn2+ Neisseria meningitidis
2.4.1.90 Fe2+ no activation Neisseria gonorrhoeae
2.4.1.90 Fe2+ partial activation Neisseria meningitidis
2.4.1.90 Mg2+ about 50% of the activity with Mn2+ Neisseria gonorrhoeae
2.4.1.90 Mg2+ about 50% of the activity with Mn2+ Neisseria meningitidis
2.4.1.90 Mn2+ required Neisseria gonorrhoeae
2.4.1.90 Mn2+ required Neisseria meningitidis
2.4.1.90 Mn2+ no activation in presence of Fe2+, Zn2+ and Cu2+ Neisseria gonorrhoeae
2.4.1.90 Mn2+ no activation in presence of Fe2+, Zn2+ and Cu2+ Neisseria meningitidis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.38 Neisseria gonorrhoeae
-
 ATCC 31151
-
2.4.1.38 Neisseria meningitidis
-
 ATCC 13102
-
2.4.1.90 Neisseria gonorrhoeae
-
 ATCC 31151
-
2.4.1.90 Neisseria meningitidis
-
 ATCC 13102
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.38 recombinant enzyme Neisseria gonorrhoeae
2.4.1.38 recombinant enzyme Neisseria meningitidis
2.4.1.90 recombinant enzyme Neisseria gonorrhoeae
2.4.1.90 recombinant enzyme Neisseria meningitidis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.38 6.5 7
-
 Neisseria gonorrhoeae
2.4.1.38 6.5 7
-
 Neisseria meningitidis
2.4.1.90 6.5 7
-
 Neisseria gonorrhoeae
2.4.1.90 6.5 7
-
 Neisseria meningitidis