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Literature summary extracted from

  • Ishibashi, T.; Ohkubo, I.; Makita, A.
    Solubilization and partial characterization of UDP-N-acetylgalactosamine: globoside alpha-N-acetylgalactosaminyltransferase from dog spleen microsomes (1977), Biochim. Biophys. Acta, 484, 24-34.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.88 diphosphatidylglycerol activation Canis lupus familiaris
2.4.1.88 GSH slight stimulation Canis lupus familiaris
2.4.1.88 additional information requirement for phospholipids Canis lupus familiaris
2.4.1.88 phosphatidic acid activation Canis lupus familiaris
2.4.1.88 phosphatidylcholine activation Canis lupus familiaris
2.4.1.88 phosphatidylethanolamine activation Canis lupus familiaris
2.4.1.88 phosphatidylinositol activation Canis lupus familiaris
2.4.1.88 phosphatidylserine activation Canis lupus familiaris
2.4.1.88 sphingomyelin activation Canis lupus familiaris
2.4.1.88 Triton X-100 activation Canis lupus familiaris
2.4.1.88 Triton X-100 best at 0.3-0.6% Canis lupus familiaris

General Stability

EC Number General Stability Organism
2.4.1.88 phosphatidylserine stabilizes Canis lupus familiaris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.88 ADP
-
 Canis lupus familiaris
2.4.1.88 ATP
-
 Canis lupus familiaris
2.4.1.88 CDP
-
 Canis lupus familiaris
2.4.1.88 EDTA strong Canis lupus familiaris
2.4.1.88 GDP
-
 Canis lupus familiaris
2.4.1.88 IDP
-
 Canis lupus familiaris
2.4.1.88 additional information no inhibitor: NaF Canis lupus familiaris
2.4.1.88 NaN3 weak Canis lupus familiaris
2.4.1.88 PCMB over 90% inhibition at 2 mM Canis lupus familiaris
2.4.1.88 UDP 85% inhibition at 1.5 mM Canis lupus familiaris
2.4.1.88 uridine weak Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.88 0.01
-
 UDP-N-acetylgalactosamine
-
 Canis lupus familiaris
2.4.1.88 0.5
-
 globoside
-
 Canis lupus familiaris

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.88 membrane
-
 Canis lupus familiaris 16020
-
2.4.1.88 microsome
-
 Canis lupus familiaris
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.88 Mg2+ no effect Canis lupus familiaris
2.4.1.88 Mn2+ optimal concentration: 10 mM Canis lupus familiaris
2.4.1.88 Mn2+ absolute requirement, cannot be replaced by Ca2+ and Mg2+ Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide Canis lupus familiaris involved in biosynthesis of carbohydrate moiety of sphingoglycolipids UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.88 Canis lupus familiaris
-
 mongrel dog, adult
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.88 partial Canis lupus familiaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.88 spleen
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.88 additional information
-
-
 Canis lupus familiaris

Storage Stability

EC Number Storage Stability Organism
2.4.1.88 -20°C, at least 2 weeks without loss of activity Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.88 UDP-N-acetyl-alpha-D-galactosamine + globoside
-
 Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 ?
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide
-
 Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?
2.4.1.88 UDP-N-acetyl-D-galactosamine + N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide involved in biosynthesis of carbohydrate moiety of sphingoglycolipids Canis lupus familiaris UDP + N-acetyl-D-galactosaminyl-1,3-N-acetyl-D-galactosaminyl-1,3-D-galactosyl-1,4-D-galactosyl-1,4-D-glucosylceramide i.e. Forssman hapten ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.88 37
-
 assay at Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.88 6.6
-
-
 Canis lupus familiaris