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Literature summary extracted from

  • Stults, C.L.M.; Macher, B.A.; Bhatti, R.; Srivastava, O.P.; Hindsgaul, O.
    Characterization of the substrate specificity of alpha1,3-galactosyltransferase utilizing modified N-acetyllactosamine disaccharides (1999), Glycobiology, 9, 661-668.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.87 Triton X-100 activates Mus musculus

Application

EC Number Application Comment Organism
2.4.1.87 synthesis useful tool for production of a range of compounds to further investigate the binding site of anti-Gal and other proteins with Galalpha(1-3)Gal binding sites Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.87 expression in COS-7 cells Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.87 diethyl dicarbonate
-
 Mus musculus
2.4.1.87 EDTA
-
 Mus musculus
2.4.1.87 Galbeta(1-3)GlcNAc-C8 strong Mus musculus
2.4.1.87 octyl 3-amino-3-deoxy-3-N-(2-diazo-3,3,3-trifluoropropionyl-beta-D-galactopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranoside) i.e. DTFP-LacNAc-C8 Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.87 additional information
-
 additional information Km-values for substituted LacNAc-saccharides containing Galalpha(1-3)Gal epitope Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.87 membrane bound Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.87 Co2+ slight activation Mus musculus
2.4.1.87 Mg2+ slight activation Mus musculus
2.4.1.87 Mn2+ pronounced requirement Mus musculus
2.4.1.87 Zn2+ slight activation Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.87 Mus musculus
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.87 additional information
-
 scale up, recombinant enzyme Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.87 UDP-galactose + substituted LacNAc-saccharides containing Galalpha(1-3)Gal epitope acceptor substrate substrate specificity, overview Mus musculus ?
-
 ?
2.4.1.87 UDP-galactose + substituted LacNAc-saccharides containing Galalpha(1-3)Gal epitope strictly specific for UDP-galactose Mus musculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.87 alpha-1,3-galactosyltransferase
-
 Mus musculus
2.4.1.87 alpha1,3GT
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.87 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.87 6
-
-
 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.87 0.27
-
 Galbeta(1-3)GlcNAc-C8
-
 Mus musculus
2.4.1.87 0.3
-
 octyl 3-amino-3-deoxy-3-N-(2-diazo-3,3,3-trifluoropropionyl-beta-D-galactopyranosyl-(1,4)-2-acetamido-2-deoxy-beta-D-glucopyranoside)
-
 Mus musculus