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Literature summary extracted from

  • Elices, M.J.; Blake, D.A.; Goldstein, I.J.
    Purification and characterization of a UDP-Gal:beta-D-Gal(1,4)-D-GlcNAc alpha(1,3)-galactosyltransferase from Ehrlich ascites tumor cells (1986), J. Biol. Chem., 261, 6064-6072.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.87 Triton X-100 absolute requirement, maximal activity at 0.5-1.0% v/v Mus musculus

General Stability

EC Number General Stability Organism
2.4.1.87 detergent: rapid and irreversible inactivation in absence of detergent, more than 50% loss of activity after 4-6 weeks Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.87 0.1
-
 beta-D-Gal(1-4)-D-GlcNAc(1-2)-[beta-D-Gal(1-4)beta-D-GlcNAc(1-6)]-D-Man
-
 Mus musculus
2.4.1.87 0.25
-
 beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man
-
 Mus musculus
2.4.1.87 0.31
-
 beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man
-
 Mus musculus
2.4.1.87 1.15
-
 beta-D-Gal(1-4)-D-GlcNAc
-
 Mus musculus
2.4.1.87 12.6
-
 UDP-galactose
-
 Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.87 membrane
-
 Mus musculus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.87 Mn2+ required Mus musculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.87 80000
-
 x * 80000, SDS-PAGE Mus musculus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.87 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.87 Ehrlich ascites carcinoma cells Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.87 Ehrlich ascites carcinoma cell
-
 Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.87 0.001
-
 membrane fraction Mus musculus
2.4.1.87 265
-
 purified enzyme Mus musculus

Storage Stability

EC Number Storage Stability Organism
2.4.1.87 -20°C, 20 mM MES, pH 6.2, 50 mM NaCl, detergent, glycerol, stable for several months Mus musculus
2.4.1.87 0°C, more than 50% loss of activity after 4-6 weeks Mus musculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.87 additional information acceptor substrate specificity Mus musculus ?
-
 ?
2.4.1.87 UDP-galactose + beta-D-Gal(1-4)-D-GlcNAc(1-2)-[beta-D-Gal(1-4)beta-D-GlcNAc(1-6)]-D-Man
-
 Mus musculus ?
-
 ?
2.4.1.87 UDP-galactose + beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man
-
 Mus musculus UDP + alpha-D-Gal(1-3)beta-D-Gal(1-4)beta-D-GlcNAc(1-2)-D-Man
-
 ?
2.4.1.87 UDP-galactose + beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man
-
 Mus musculus UDP + alpha-D-Gal(1-3)beta-D-Gal(1-4)beta-D-GlcNAc(1-6)-D-Man
-
 ?
2.4.1.87 UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 ?
2.4.1.87 UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R not: alpha-L-Fuc(1-2)beta-D-Gal(1-4)-D-GlcNAc Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.87 UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R beta-D-Gal(1-4)-[alpha-L-Fuc(1-3)]-D-GlcNAc Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.87 UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R very poor acceptor: lactose, beta-D-Gal(1-3)-D-GlcNAc Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.87 UDP-galactose + lactose very poor acceptor Mus musculus UDP + alpha-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-glucose
-
 ?
2.4.1.87 UDP-galactose + N-acetyllactosamine oligosaccharides, glycoproteins and glycosaminoglycans containing the terminal nonreducing N-acetyllactosamine unit all serve as acceptors Mus musculus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.87 ? x * 80000, SDS-PAGE Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.87 37
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.87 6.2
-
-
 Mus musculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.87 5.3 7 pH 5.3: about 45% of activity maximum, pH 7.0: about 30% of activity maximum Mus musculus