Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Van den Eijnden, D.H.; Blanken, W.M.; Winterwerp, H.; Schiphorst, W.E.C.M.
    Identification and characterization of an UDP-Gal: N-acetyllactosaminide alpha-1,3-D-galactosyltransferase in calf thymus (1983), Eur. J. Biochem., 134, 523-530.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.87 Triton X-100 activates Bos taurus
2.4.1.87 Triton X-100 at 0.8% v/v 4fold stimulation Bos taurus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.87 EDTA weak Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.87 0.2
-
 UDP-galactose
-
 Bos taurus
2.4.1.87 2.7
-
 N-acetyllactosamine
-
 Bos taurus
2.4.1.87 3.7
-
 asialo-alpha1-acid glycoprotein
-
 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.87 membrane bound Bos taurus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.87 Mg2+ slight activation Bos taurus
2.4.1.87 Mn2+ Km 6.1 mM Bos taurus
2.4.1.87 Mn2+ pronounced requirement Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.87 additional information Bos taurus functions in the biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins ?
-
 ?
2.4.1.87 additional information Bos taurus functions in biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.87 Bos taurus
-
 calf
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.87 thymus
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.87 additional information functions in the biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins Bos taurus ?
-
 ?
2.4.1.87 additional information functions in biosynthesis of calf thymocyte cell-surface glycoconjugates including glycoproteins Bos taurus ?
-
 ?
2.4.1.87 UDP-galactose + asialo-alpha1-acid glycoprotein
-
 Bos taurus UDP + alpha-D-galactosyl-asialo-alpha1-acid glycoprotein
-
 ?
2.4.1.87 UDP-galactose + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R enzyme introduces galactosyl residues in alpha anomeric configuration to the Galbeta(1-4)GlcNAc units on the acceptor substrate Bos taurus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
2.4.1.87 UDP-galactose + glycoprotein the nonreducing terminal N-acetyllactosamine residue of glycoproteins can act as acceptor Bos taurus UDP + alpha-D-galactosylglycoprotein
-
 ?
2.4.1.87 UDP-galactose + lactose
-
 Bos taurus UDP + alpha-D-galactosyl-(1-3)-beta-D-galactosyl-(1-4)-beta-D-glucose
-
 ?
2.4.1.87 UDP-galactose + N-acetyllactosamine i.e. beta-D-galactosyl-1,4-N-acetyl-D-glucosamine Bos taurus UDP + alpha-D-galactosyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosamine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.87 37
-
 assay at Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.87 5.5 7 cacodylate buffer Bos taurus
2.4.1.87 6
-
 acetate buffer Bos taurus