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Literature summary extracted from

  • Carlo, P.L.; Villemez, C.L.
    Solubilization and properties of polyprenyl phosphate: GDP-D-mannose mannosyl transferase (1979), Arch. Biochem. Biophys., 198, 117-123.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.83 CuSO4
-
 Acanthamoeba castellanii
2.4.1.83 GDP 0.01 mM, 81% inhibition Acanthamoeba castellanii
2.4.1.83 GDP-D-glucose 0.01 mM, 50% inhibition Acanthamoeba castellanii
2.4.1.83 GMP 1 mM, 84% inhibition; competitive Acanthamoeba castellanii
2.4.1.83 HgCl2
-
 Acanthamoeba castellanii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.00025
-
 GDPmannose
-
 Acanthamoeba castellanii
2.4.1.83 0.0033
-
 dolichyl phosphate
-
 Acanthamoeba castellanii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.83 Co2+ less efficient in activation than Mn2+ Acanthamoeba castellanii
2.4.1.83 Mg2+ less efficient than Mn2+ in activation Acanthamoeba castellanii
2.4.1.83 Mn2+ divalent cation required, maximal activity with Mn2+ Acanthamoeba castellanii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Acanthamoeba castellanii
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.83 cell culture
-
 Acanthamoeba castellanii
-
2.4.1.83 cell culture encysting Acanthamoeba castellanii
-
2.4.1.83 cyst
-
 Acanthamoeba castellanii
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.83 -20°C, loss of a third of the activity after 2 weeks Acanthamoeba castellanii
2.4.1.83 -70°C, 2 weeks, no loss of activity Acanthamoeba castellanii
2.4.1.83 0°C, 3 days, 50% loss of activity Acanthamoeba castellanii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDPmannose + dolichyl phosphate
-
 Acanthamoeba castellanii GDP + dolichyl D-mannosyl phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.83 27
-
-
 Acanthamoeba castellanii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.83 15 35 15°C: about 60% of maximal activity, 35°C: about 30% of maximal activity Acanthamoeba castellanii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.83 7
-
-
 Acanthamoeba castellanii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.83 5.8 8 pH 5.8: about 35% of maximal activity, pH 8.0: about 60% of maximal activity Acanthamoeba castellanii