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Literature summary extracted from

  • Jensen, J.W.; Schutzbach, J.S.
    Characterization of mannosyl-transfer reactions catalyzed by dolichyl-mannosyl-phosphate-synthase (1986), Carbohydr. Res., 149, 199-208.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.83 Phospholipid no absolute requirement for phospholipid, the phospholipid is required for interaction of the enzyme with the long chain polyisoprenol substrate dolichyl phosphate Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.83 Amphomycin 0.03 mg/ml, 50% inhibition Rattus norvegicus
2.4.1.83 GDP 0.0013 mM, 50% inhibition Rattus norvegicus
2.4.1.83 GMP 0.016 mM, 50% inhibition Rattus norvegicus
2.4.1.83 GTP 0.003 mM, 50% inhibition Rattus norvegicus
2.4.1.83 Nonidet P-40 0.01%, 50% inhibition Rattus norvegicus
2.4.1.83 p-hydroxymercuribenzoate 0.003 mM, 50% inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.83 0.0003
-
 dolichyl phosphate
-
 Rattus norvegicus
2.4.1.83 0.00069
-
 GDPmannose
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.83 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.83 Co2+ 5 mM, divalent metal required, 90% of the activation with 5 mM Mn2+ Rattus norvegicus
2.4.1.83 Mg2+ 5 mM, divalent metal required, 70% of the activation with 5 mM Mn2+ Rattus norvegicus
2.4.1.83 Mn2+ 5 mM, divalent cation required, highest activation with Mn2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.83 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.83 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.83 GDPmannose + dolichyl phosphate
-
 Rattus norvegicus GDP + dolichyl D-mannosyl phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.83 7.5
-
-
 Rattus norvegicus