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Literature summary extracted from

  • Marks, D.L.; Paul, P.; Kamisaka, Y.; Pagano, R.E.
    Methods for studying glucosylceramide synthase (2000), Methods Enzymol., 311, 50-59.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.80 medicine
-
 Homo sapiens
2.4.1.80 medicine enzyme inhibition is a possible target for chemotherapeutic agents for a number of diseases, including cancer Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.80 cloning from rat brain genetic library and expression in Escherichia coli BL21(DE3) Rattus norvegicus
2.4.1.80 functional expression in Escherichia coli BL21(DE3) Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.80 EDTA no inhibition Rattus norvegicus
2.4.1.80 additional information
-
 Gallus gallus
2.4.1.80 additional information
-
 Homo sapiens
2.4.1.80 additional information no inhibition by DTT Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.80 Golgi membrane integral membrane protein Sus scrofa 139
-
2.4.1.80 Golgi membrane orientation to the cytosolic side Rattus norvegicus 139
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.80 Ca2+ stimulates Mus musculus
2.4.1.80 Ca2+ stimulates Homo sapiens
2.4.1.80 Ca2+ stimulates Rattus norvegicus
2.4.1.80 Mg2+ stimulates Mus musculus
2.4.1.80 Mg2+ stimulates Homo sapiens
2.4.1.80 Mg2+ stimulates Rattus norvegicus
2.4.1.80 Mg2+ stimulates Sus scrofa
2.4.1.80 Mn2+ stimulates Mus musculus
2.4.1.80 Mn2+ stimulates Homo sapiens
2.4.1.80 Mn2+ stimulates Rattus norvegicus
2.4.1.80 Mn2+ stimulates Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.80 additional information
-
-
 Mus musculus
2.4.1.80 additional information
-
-
 Homo sapiens
2.4.1.80 additional information
-
-
 Rattus norvegicus
2.4.1.80 additional information
-
 enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Homo sapiens
2.4.1.80 additional information
-
 enzyme forms dimeric or oligomeric complexes with another protein in the Golgi membrane vivo, topology Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine Gallus gallus
-
 UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine Homo sapiens
-
 UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine Sus scrofa
-
 UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine Mus musculus
-
 UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine Rattus norvegicus
-
 UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine Homo sapiens
-
 UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine Rattus norvegicus Sprague-Dawley
-
 UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.80 Gallus gallus
-
-
-
2.4.1.80 Homo sapiens
-
-
-
2.4.1.80 Homo sapiens Q16739
-
-
2.4.1.80 Mus musculus
-
-
-
2.4.1.80 Rattus norvegicus
-
 Sprague-Dawley
-
2.4.1.80 Rattus norvegicus Q9R0E0
-
-
2.4.1.80 Rattus norvegicus Sprague-Dawley
-
 Sprague-Dawley
-
2.4.1.80 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.80 partial from Golgi membranes Rattus norvegicus
2.4.1.80 solubilization method Rattus norvegicus
2.4.1.80 solubilization method Sus scrofa

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.4.1.80 reconstitution of solubilized enzyme in detergent-free lipid vesicles Rattus norvegicus
2.4.1.80 reconstitution of solubilized enzyme in detergent-free lipid vesicles Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.80 keratinocyte
-
 Homo sapiens
-
2.4.1.80 liver
-
 Mus musculus
-
2.4.1.80 liver
-
 Homo sapiens
-
2.4.1.80 liver low level Rattus norvegicus
-
2.4.1.80 lung
-
 Rattus norvegicus
-
2.4.1.80 lung
-
 Homo sapiens
-
2.4.1.80 spleen
-
 Rattus norvegicus
-
2.4.1.80 testis
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.80 additional information
-
-
 Gallus gallus
2.4.1.80 additional information
-
-
 Mus musculus
2.4.1.80 additional information
-
-
 Homo sapiens
2.4.1.80 additional information
-
-
 Sus scrofa
2.4.1.80 additional information
-
 fluorescence assay method Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Gallus gallus UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Homo sapiens UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Sus scrofa UDP + D-glucosyl-N-acylsphingosine
-
 ?
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Mus musculus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Homo sapiens UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Gallus gallus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Mus musculus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Homo sapiens UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Sus scrofa UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine
-
 Rattus norvegicus Sprague-Dawley UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Rattus norvegicus Sprague-Dawley UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay Rattus norvegicus UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
 ?
2.4.1.80 UDP-glucose + N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine synthetic fluorescent ceramide substrate analogue for fluorescence enzyme assay Rattus norvegicus Sprague-Dawley UDP + D-glucosyl-N-[7-(4-nitrobenzo-2-oxa-1,3-diazole)]-6-aminocaproyl-D-erythro-sphingosine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.80 GCS
-
 Mus musculus
2.4.1.80 GCS
-
 Homo sapiens
2.4.1.80 GCS
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.80 37
-
 assay at Mus musculus
2.4.1.80 37
-
 assay at Rattus norvegicus
2.4.1.80 37
-
 assay at Sus scrofa
2.4.1.80 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.80 7.4
-
-
 Mus musculus
2.4.1.80 7.4
-
-
 Homo sapiens
2.4.1.80 7.4
-
 assay at Rattus norvegicus