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Literature summary extracted from

  • Coste, H.; Martel, M.B.; Azzar, G.; Got, R.
    UDPglucose-ceramide glucosyltransferase from porcine submaxillary glands is associated with the Golgi apparatus (1985), Biochim. Biophys. Acta, 814, 1-7.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.80 CHAPS i.e. 3-[(3-cholamidopropyl)dimethylamonio]-1propanesulfonate Sus scrofa
2.4.1.80 detergent CHAPS stimulates Sus scrofa
2.4.1.80 NADH omission in assay containing Golgi vesicles leads to loss of about 90% activity Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.80 EDTA
-
 Sus scrofa
2.4.1.80 Triton X-100
-
 Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.80 0.022
-
 UDP-glucose
-
 Sus scrofa
2.4.1.80 0.054
-
 ceramide
-
 Sus scrofa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.80 Golgi apparatus
-
 Sus scrofa 5794
-
2.4.1.80 Golgi membrane
-
 Sus scrofa 139
-
2.4.1.80 membrane
-
 Sus scrofa 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.80 Mg2+ stimulates Sus scrofa
2.4.1.80 Mn2+ stimulates Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine Sus scrofa catalyzes the first step during the sequential addition of carbohydrate moieties for ganglioside biosynthesis UDP + D-glucosyl-N-acylsphingosine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.80 Sus scrofa
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.80 submaxillary ganglion
-
 Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.80 0.0000065
-
-
 Sus scrofa
2.4.1.80 0.0007
-
 Golgi fraction Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.80 UDP-glucose + N-acylsphingosine i.e. ceramide Sus scrofa UDP + D-glucosyl-N-acylsphingosine i.e. glucosylceramide ?
2.4.1.80 UDP-glucose + N-acylsphingosine catalyzes the first step during the sequential addition of carbohydrate moieties for ganglioside biosynthesis Sus scrofa UDP + D-glucosyl-N-acylsphingosine
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.80 additional information
-
 sharp fall of activity below and above pH 6.5 Sus scrofa
2.4.1.80 6.5
-
-
 Sus scrofa