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Literature summary extracted from

  • Trinchera, M.; Bozzaro, S.
    Dictyostelium cytosolic fucosyltransferase synthesizes H type 1 trisaccharide in vitro (1996), FEBS Lett., 395, 68-72.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.69 ATP stimulates at low concentrations Dictyostelium discoideum
2.4.1.69 fucose stimulates Dictyostelium discoideum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.69 2-mercaptoethanol complete inhibition of activity with lacto-N-biose I Dictyostelium discoideum
2.4.1.69 NEM
-
 Dictyostelium discoideum
2.4.1.69 Triton X-100
-
 Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.69 0.00127
-
 GDP-L-fucose
-
 Dictyostelium discoideum
2.4.1.69 2.8
-
 lacto-N-biose I
-
 Dictyostelium discoideum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.69 cytosol
-
 Dictyostelium discoideum 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.69 Mg2+ strong stimulation by Mg2+ and Mn2+ Dictyostelium discoideum
2.4.1.69 Mn2+ strong stimulation by Mg2+ and Mn2+ Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.69 Dictyostelium discoideum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.69 GDP-L-fucose + beta-D-galactosyl-R
-
 Dictyostelium discoideum GDP + alpha-L-fucosyl-1,2-beta-D-galactosyl-R
-
 ?
2.4.1.69 GDP-L-fucose + lacto-N-biose 1
-
 Dictyostelium discoideum GDP + L-Fucalpha(1-2)Galbeta(1-3)GlcNAc
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.69 23
-
-
 Dictyostelium discoideum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.69 16 30 16°C: 61% of maximal activity, 30°C: 62% of maximal activity Dictyostelium discoideum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.69 100
-
 complete inhibition by heating at 100°C Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.69 6.9
-
-
 Dictyostelium discoideum