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Literature summary extracted from

  • Yanagidani, S.; Uozumi, N.; Ihara, Y.; Miyoshi, E.; Yamaguchi, N.; Taniguchi, N.
    Purification and cDNA cloning of GDP-L-Fuc:N-acetyl-beta-D-glucosaminide:alpha1-6 fucosyltransferase (alpha1-6 FucT) from human gastric cancer MKN45 cells (1997), J. Biochem., 121, 626-632.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.68 cloned from cDNA library of MKN45 cells, DNA sequence determination and analysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.68 Cd2+
-
 Homo sapiens
2.4.1.68 Co2+
-
 Homo sapiens
2.4.1.68 Cu2+
-
 Homo sapiens
2.4.1.68 Cu2+
-
 Sus scrofa
2.4.1.68 additional information no inhibition by EDTA, Mg2+, Ca2+ Homo sapiens
2.4.1.68 additional information no inhibition by EDTA, Mg2+, Ca2+ Sus scrofa
2.4.1.68 Ni2+
-
 Homo sapiens
2.4.1.68 Ni2+
-
 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.68 additional information
-
 amino acid sequence, alignment and comparison Sus scrofa
2.4.1.68 additional information
-
 amino acid sequence, alignment and comparison Homo sapiens
2.4.1.68 60000
-
 x * 60000, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.68 Homo sapiens
-
-
-
2.4.1.68 Homo sapiens Q9BYC5
-
-
2.4.1.68 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.68 no glycoprotein no N-glycosylation sites in predicted amino acid sequence Sus scrofa
2.4.1.68 no glycoprotein no N-glycosylation sites in predicted amino acid sequence Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.68 by affinity chromatography with GDP-hexanolamine resin and GnGn-bi-Asn-resin Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.68 brain
-
 Sus scrofa
-
2.4.1.68 MKN-45 cell
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.68 0.00000025
-
 COS-1 cells Homo sapiens
2.4.1.68 0.0000355
-
 recombinant enzyme from COS-1 cells Homo sapiens
2.4.1.68 0.497
-
 purified enzyme Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.68 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine i.e. GnGn-peptide Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
2.4.1.68 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine i.e. GnGn-peptide Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine
-
 ?
2.4.1.68 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGn-bi-Asn-PABA, synthetic substrate, fluorescent assay Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
2.4.1.68 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGn-bi-Asn-PABA, synthetic substrate, fluorescent assay Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
2.4.1.68 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) acceptor substrate is derived from bovine gamma-globulin Homo sapiens GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?
2.4.1.68 GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) acceptor substrate is derived from bovine gamma-globulin Sus scrofa GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-6)]-N-acetyl-beta-D-glucosaminyl]asparagine-bi-(4-(2-pyridylamino)butylamine) i.e. GnGnF-bi-Asn-PABA ?

Subunits

EC Number Subunits Comment Organism
2.4.1.68 ?
-
 Homo sapiens
2.4.1.68 ?
-
 Sus scrofa
2.4.1.68 ? x * 60000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
2.4.1.68 alpha1-6FucT
-
 Homo sapiens
2.4.1.68 alpha1-6FucT
-
 Sus scrofa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.68 30 37
-
 Sus scrofa
2.4.1.68 30 37
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.68 7.5
-
-
 Homo sapiens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.68 4 10
-
 Sus scrofa
2.4.1.68 4 10
-
 Homo sapiens