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Literature summary extracted from

  • Kizawa, H.; Miyagawa, K.I.; Sugiyama, Y.
    Purification and characterization of trehalose phosphorylase from Micrococcus varians (1995), Biosci. Biotechnol. Biochem., 59, 1908-1912.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.64 1-deoxynojirimycin 1 mM, complete inhibition Kocuria varians
2.4.1.64 Cu2+ 1 mM, complete inhibition Kocuria varians
2.4.1.64 Ni2+ 1 mM, 88% inhibition Kocuria varians
2.4.1.64 validamycin A 10 mM, complete inhibition Kocuria varians
2.4.1.64 validoxylamine A 0.2 mM, complete inhibition Kocuria varians
2.4.1.64 Zn2+ 1 mM, complete inhibition Kocuria varians

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.64 3.1
-
 phosphate
-
 Kocuria varians
2.4.1.64 10
-
 trehalose
-
 Kocuria varians
2.4.1.64 23
-
 glucose
-
 Kocuria varians
2.4.1.64 38
-
 beta-D-glucose 1-phosphate
-
 Kocuria varians

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.64 105000
-
 6 * 105000, SDS-PAGE Kocuria varians
2.4.1.64 570000 580000 gel filtration Kocuria varians

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.64 Kocuria varians
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.64 18.2
-
-
 Kocuria varians

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.64 alpha,alpha-trehalose + phosphate
-
 Kocuria varians D-glucose + beta-D-glucose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.64 hexamer 6 * 105000, SDS-PAGE Kocuria varians

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.64 32
-
 both directions Kocuria varians

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.64 30
-
 stable below Kocuria varians

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.1.64 200
-
 phosphate
-
 Kocuria varians
2.4.1.64 200
-
 trehalose
-
 Kocuria varians
2.4.1.64 660
-
 glucose
-
 Kocuria varians
2.4.1.64 660
-
 beta-D-glucose 1-phosphate
-
 Kocuria varians

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.64 6.3
-
 synthesis of trehalose Kocuria varians
2.4.1.64 7
-
 phosphorolysis of trehalose Kocuria varians

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.64 5.5 7.5 stable Kocuria varians