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Literature summary extracted from

  • Marechal, L.R.; Belocopitow, E.
    Metabolism of trehalose in Euglena gracilis. I. Partial purification and some properties of trehalose phosphorylase (1972), J. Biol. Chem., 247, 3223-3228.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.64 enzyme retains full activity during 5 months at -14°C with several freezings and thawings, in presence of 2 mM phosphate buffer, pH 7.0, beta-D-glucose-1-phosphate and alpha-D-glucose-1-phosphate Euglena gracilis
2.4.1.64 loss of activity when extracts are desalted by dialysis or passage through Sephadex G-25 in presence of water. When dialysis is carried out against 5 mM NaCl, glucose 6-phosphate, EDTA, or imidazole-HCl buffer, pH 7.0, the enzymatic activity is maintained only for a few h Euglena gracilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.64 6
-
 beta-D-glucose 1-phosphate
-
 Euglena gracilis
2.4.1.64 9.4
-
 phosphate
-
 Euglena gracilis
2.4.1.64 32
-
 glucose
-
 Euglena gracilis
2.4.1.64 33
-
 trehalose
-
 Euglena gracilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.64 344000
-
 sucrose density gradient centrifugation Euglena gracilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.64 alpha,alpha-trehalose + phosphate Euglena gracilis enzyme is involved in trehalose catabolism D-glucose + beta-D-glucose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.64 Euglena gracilis
-
 var. bacillaris
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.64 partial Euglena gracilis

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.64 0.214
-
-
 Euglena gracilis

Storage Stability

EC Number Storage Stability Organism
2.4.1.64 14°C,in presence of 2 mM phosphate buffer, pH 7.0, beta-D-glucose-1-phosphate and alpha-D-glucose-1-phosphate, enzyme retains full activity during 5 months with several freezings and thawings Euglena gracilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.64 6-deoxy-D-glucose + alpha-D-glucose 1-phosphate at 93% the activity with rate of glucose glucosylation Euglena gracilis alpha-D-glucopyranosyl 6-deoxy-alpha-D-glucopyranoside + phosphate
-
 ?
2.4.1.64 alpha,alpha-trehalose + phosphate
-
 Euglena gracilis D-glucose + beta-D-glucose 1-phosphate
-
 r
2.4.1.64 alpha,alpha-trehalose + phosphate enzyme is involved in trehalose catabolism Euglena gracilis D-glucose + beta-D-glucose 1-phosphate
-
 ?
2.4.1.64 xylose + beta-D-glucose 1-phosphate glucosylated at 23% of the rate of glucose glucosylation Euglena gracilis glucosyl-1,1-xylose 1-phosphate
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.64 6.3
-
 synthesis of trehalose Euglena gracilis
2.4.1.64 7
-
-
 Euglena gracilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.64 5.7 7.8 about half-maximal activity at pH 5.7 and 7.8, trehalose synthesis Euglena gracilis
2.4.1.64 6 8 about half-maximal activity at pH 6 and 8, phosphorolysis Euglena gracilis