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Literature summary extracted from

  • Rothfield, L.; Osborn, M.J.; Horecker, B.L.
    Biosynthesis of bacterial lipopolysaccharide. II. Incorporation of glucose and galactose catalyzed by particulate and soluble enzymes in Salmonella (1964), J. Biol. Chem., 239, 2788-2795.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.58 freezing and thawing: rapid loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.58 0.01
-
 lipopolysaccharide
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 0.074
-
 UDPglucose
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.58 additional information most of the activity is located in the cell-wall fraction, but also significant activity in soluble fraction Salmonella enterica subsp. enterica serovar Typhimurium
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.58 Mg2+ soluble enzyme: MgCl2 stimulates more than MnCl2, optimal concentration: 0.02 M Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58 Mn2+ soluble enzyme: MgCl2 stimulates more than MnCl2 Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.58 UDPglucose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium involved in biosynthesis of cell wall lipopolysaccharide UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium SL797 involved in biosynthesis of cell wall lipopolysaccharide UDP + D-glucosyllipopolysaccharide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.58 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.4.1.58 Salmonella enterica subsp. enterica serovar Typhimurium SL797
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.58 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.4.1.58 -18°C or 4°C, soluble enzyme, overnight storage, 30-50% loss of activity Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.58 additional information no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.1.58 additional information no sugar donors: UDPgalactose, TDPglucose, glucose, glucose-1-phosphate Salmonella enterica subsp. enterica serovar Typhimurium SL797 ?
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide UDPglucose is the only effective glucosyl donor Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide transfers glucosyl residues to the backbone portion of lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide involved in biosynthesis of cell wall lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide UDPglucose is the only effective glucosyl donor Salmonella enterica subsp. enterica serovar Typhimurium SL797 UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide transfers glucosyl residues to the backbone portion of lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium SL797 UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide transfers glucose only to specific glucose-acceptor sites in the glucose-deficient core polysaccharide Salmonella enterica subsp. enterica serovar Typhimurium SL797 UDP + D-glucosyllipopolysaccharide
-
 ?
2.4.1.58 UDPglucose + lipopolysaccharide involved in biosynthesis of cell wall lipopolysaccharide Salmonella enterica subsp. enterica serovar Typhimurium SL797 UDP + D-glucosyllipopolysaccharide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.58 7.8
-
 soluble enzyme, Tris buffer Salmonella enterica subsp. enterica serovar Typhimurium