BRENDA - Enzyme Database

Studies on the biosynthesis of mannan in Micrococcus lysodeikticus. II. The enzymatic synthesis of mannosyl-l-phosphoryl-undecaprenol

Lahav, M.; Chiu, T.H.; Lennarz, W.J.; J. Biol. Chem. 244, 5890-5898 (1969)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.54
Cutscum
activates
Micrococcus luteus
2.4.1.54
Detergents
activate
Micrococcus luteus
2.4.1.54
Nonidet P-40
activates
Micrococcus luteus
2.4.1.54
phosphatidylethanolamine
activates
Micrococcus luteus
2.4.1.54
phosphatidylglycerol
requirement
Micrococcus luteus
2.4.1.54
sodium lauryl sulfate
activates
Micrococcus luteus
2.4.1.54
Triton X-100
activates
Micrococcus luteus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.54
GDP
-
Micrococcus luteus
2.4.1.54
phosphatidylglycerol
inhibits in excess amount
Micrococcus luteus
2.4.1.54
undecaprenyl phosphate
inhibits in excess amount
Micrococcus luteus
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.54
Ca2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
2.4.1.54
Mg2+
optimal concentration: 67 mM; requirement
Micrococcus luteus
2.4.1.54
Mn2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
2.4.1.54
additional information
not activated by Co2+
Micrococcus luteus
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.54
additional information
Micrococcus luteus
involved in biosynthesis of mannan
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.54
Micrococcus luteus
-
Micrococcus lysodeikticus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.54
GDPmannose + ficaprenyl phosphate
effective substrate
489288
Micrococcus luteus
GDP + mannosyl-1-phosphorylficaprenol
-
489288
Micrococcus luteus
?
2.4.1.54
GDPmannose + undecaprenyl phosphate
-
489288
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
-
489288
Micrococcus luteus
r
2.4.1.54
additional information
involved in biosynthesis of mannan
489288
Micrococcus luteus
?
-
-
-
-
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.54
37
-
assay at
Micrococcus luteus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.54
6.9
7.5
0.06 M Tris-maleate buffer
Micrococcus luteus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.54
6.2
8.6
about half-maximal activity at pH 6.2 and 8.6
Micrococcus luteus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.54
Cutscum
activates
Micrococcus luteus
2.4.1.54
Detergents
activate
Micrococcus luteus
2.4.1.54
Nonidet P-40
activates
Micrococcus luteus
2.4.1.54
phosphatidylethanolamine
activates
Micrococcus luteus
2.4.1.54
phosphatidylglycerol
requirement
Micrococcus luteus
2.4.1.54
sodium lauryl sulfate
activates
Micrococcus luteus
2.4.1.54
Triton X-100
activates
Micrococcus luteus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.54
GDP
-
Micrococcus luteus
2.4.1.54
phosphatidylglycerol
inhibits in excess amount
Micrococcus luteus
2.4.1.54
undecaprenyl phosphate
inhibits in excess amount
Micrococcus luteus
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.54
Ca2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
2.4.1.54
Mg2+
optimal concentration: 67 mM; requirement
Micrococcus luteus
2.4.1.54
Mn2+
activation, can replace Mg2+ to some extent
Micrococcus luteus
2.4.1.54
additional information
not activated by Co2+
Micrococcus luteus
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.54
additional information
Micrococcus luteus
involved in biosynthesis of mannan
?
-
-
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.54
GDPmannose + ficaprenyl phosphate
effective substrate
489288
Micrococcus luteus
GDP + mannosyl-1-phosphorylficaprenol
-
489288
Micrococcus luteus
?
2.4.1.54
GDPmannose + undecaprenyl phosphate
-
489288
Micrococcus luteus
GDP + D-mannosyl-1-phosphoundecaprenol
-
489288
Micrococcus luteus
r
2.4.1.54
additional information
involved in biosynthesis of mannan
489288
Micrococcus luteus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.54
37
-
assay at
Micrococcus luteus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.54
6.9
7.5
0.06 M Tris-maleate buffer
Micrococcus luteus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
2.4.1.54
6.2
8.6
about half-maximal activity at pH 6.2 and 8.6
Micrococcus luteus