EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.50 | dithiothreitol | 20 mM, activates | Gallus gallus | |
2.4.1.50 | Nonidet P-40 | activates | Gallus gallus | |
2.4.1.50 | Triton X-100 | 0.1%, 2fold stimulation | Gallus gallus | |
2.4.1.50 | Urea | 2 mM, activates | Gallus gallus |
EC Number | General Stability | Organism |
---|---|---|
2.4.1.50 | highly labile enzyme, DTT stabilizes during purification, 0.25 M or 0.1 M galactose or glycerol 25% v/v do not stabilize | Gallus gallus |
2.4.1.50 | unstable during purification | Gallus gallus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.50 | ADP | - |
Gallus gallus | |
2.4.1.50 | AMP | - |
Gallus gallus | |
2.4.1.50 | ATP | - |
Gallus gallus | |
2.4.1.50 | CDP | - |
Gallus gallus | |
2.4.1.50 | Co2+ | 6 mM: 50% inhibition | Gallus gallus | |
2.4.1.50 | concanavalin A | 0.3 M methyl-alpha-D-glucopyranoside protects, but not galactose | Gallus gallus | |
2.4.1.50 | Galactosylhydroxylysine | - |
Gallus gallus | |
2.4.1.50 | GDP | - |
Gallus gallus | |
2.4.1.50 | gelatinized calf skin collagen | above 60 mg/ml | Gallus gallus | |
2.4.1.50 | human renal basement membrane collagen | glucose-free | Gallus gallus | |
2.4.1.50 | hydroxylysine | weak | Gallus gallus | |
2.4.1.50 | Mn2+ | above 2.5 mM | Gallus gallus | |
2.4.1.50 | additional information | not inhibited by lysine, free monosaccharides, 1 mM lactose, 1 mM sucrose, 2 mM uridine | Gallus gallus | |
2.4.1.50 | UDP | - |
Gallus gallus | |
2.4.1.50 | UDPgalacturonate | - |
Gallus gallus | |
2.4.1.50 | UDPglucose | - |
Gallus gallus | |
2.4.1.50 | UDPglucuronate | - |
Gallus gallus | |
2.4.1.50 | UMP | - |
Gallus gallus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.50 | additional information | - |
additional information | Km: 16 mg/ml, gelatinized calf skin collagen | Gallus gallus | |
2.4.1.50 | 0.02 | 0.023 | UDPgalactose | - |
Gallus gallus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.50 | endoplasmic reticulum cisterna | presumed location within | Gallus gallus | - |
- |
2.4.1.50 | membrane | associated with membrane structures | Gallus gallus | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.50 | Mn2+ | requirement | Gallus gallus | |
2.4.1.50 | Mn2+ | optimal concentration: 2-2.5 mM | Gallus gallus | |
2.4.1.50 | additional information | not activated by Co2+ | Gallus gallus | |
2.4.1.50 | additional information | not activated by Mg2+ | Gallus gallus | |
2.4.1.50 | additional information | not activated by Fe2+ | Gallus gallus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.50 | additional information | Gallus gallus | collagen biosynthesis | ? | - |
? |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.4.1.50 | Ethylene glycol | 50% v/v, stable to | Gallus gallus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.50 | Gallus gallus | - |
white Leghorn chicken | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.4.1.50 | glycoprotein | presence of carbohydrate units in enzyme molecule | Gallus gallus |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.50 | 250-300fold | Gallus gallus |
2.4.1.50 | optimal extraction requires high concentrations of detergent and salt | Gallus gallus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.50 | embryo | 14-15 days old | Gallus gallus | - |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.50 | -20°C, 50% v/v ethylene glycol, several weeks, stable | Gallus gallus |
2.4.1.50 | 4°C, unstable to storage, urea, galactose, Triton X-100, glycerol or ethylene glycol do not stabilize | Gallus gallus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.50 | additional information | no acceptor: free hydroxylysine | Gallus gallus | ? | - |
? | |
2.4.1.50 | additional information | collagen biosynthesis | Gallus gallus | ? | - |
? | |
2.4.1.50 | UDPgalactose + procollagen 5-hydroxy-L-lysine | gelatinized insoluble calf skin collagen | Gallus gallus | UDP + procollagen 5-(D-galactosyloxy)-L-lysine | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.50 | 37 | - |
assay at | Gallus gallus |