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Literature summary extracted from

  • Barber, A.J.; Jamieson, G.A.
    Characterization of membrane-bound collagen galactosyltransferase of human blood platelets (1971), Biochim. Biophys. Acta, 252, 546-552.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.50 Triton X-100 0.1%, 2fold stimulation Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.50 Cu2+
-
 Homo sapiens
2.4.1.50 additional information not inhibited by Hg2+, acetylsalicylic acid, D-glucosamine, glutathione, ADP Homo sapiens
2.4.1.50 p-hydroxymercuribenzoate 10 mM, 50% inhibition Homo sapiens
2.4.1.50 Pb2+
-
 Homo sapiens
2.4.1.50 UDP
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.50 plasma membrane blood platelet plasma membrane, may be buried in the interior of the membrane or located on its inner surface Homo sapiens 5886
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.50 Ca2+ 20% of activation by Mg2+ Homo sapiens
2.4.1.50 Cd2+ 20% of activation by Mg2+ Homo sapiens
2.4.1.50 Co2+ 50% of activation by Mg2+ Homo sapiens
2.4.1.50 Mg2+ 50% of activation by Mg2+ Homo sapiens
2.4.1.50 Mn2+ requirement Homo sapiens
2.4.1.50 Mn2+ optimal concentration: 15 mM Homo sapiens
2.4.1.50 additional information not activated by Cu2+ Homo sapiens
2.4.1.50 additional information not activated by Zn2+ Homo sapiens
2.4.1.50 additional information not activated by Hg2+ Homo sapiens
2.4.1.50 additional information not activated by K+, Pb2+ Homo sapiens
2.4.1.50 additional information not activated by Fe3+, Ba2+ Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine Homo sapiens incorporation of monosaccharide precursors into the heterosaccharide chains of collagen UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.50 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.50 blood platelet blood platelet plasma membranes Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.50 additional information
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.50 additional information no sugar donors: ADP-glucose, UDP-N-acetylglucosamine, UDP-N-acetylgalactosamine Homo sapiens ?
-
 ?
2.4.1.50 additional information no sugar donor: UDPglucose Homo sapiens ?
-
 ?
2.4.1.50 additional information no acceptors: transferrin, native fetuin Homo sapiens ?
-
 ?
2.4.1.50 UDPgalactose + fetuin with sialic acid and galactose removed 33% of activity with bovine achilles tendon collagen with glucose and galactose residues removed Homo sapiens ?
-
 ?
2.4.1.50 UDPgalactose + ovalbumin 17.9% of activity with bovine achilles tendon collagen with glucose and galactose residues removed Homo sapiens ?
-
 ?
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine bovine achilles tendon collagen with glucose and galactose residues removed Homo sapiens UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine high specificity for specific hydroxylysine residues in collagen Homo sapiens UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
2.4.1.50 UDPgalactose + procollagen 5-hydroxy-L-lysine incorporation of monosaccharide precursors into the heterosaccharide chains of collagen Homo sapiens UDP + procollagen 5-(D-galactosyloxy)-L-lysine
-
 ?
2.4.1.50 UDPgalactose + submaxillary glycoprotein with sialic acid removed bovine submaxillary glycoprotein with sialic acid removed, 9.5% of activity with achilles tendon collagen with glucose and galactose residues removed Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.50 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.50 7.5
-
-
 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.50 5.5 9.5 about half-maximal activity at pH 5.5 and 9.5 Homo sapiens