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Literature summary extracted from
- Purification and properties of a cellobiose phosphorylase (CepA) and a cellodextrin phosphorylase (CepB) from the cellulolytic thermophile Clostridium stercorarium (1997), Eur. J. Biochem., 247, 262-267.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 2.4.1.20 | - |
Thermoclostridium stercorarium |
| 2.4.1.49 | DNA sequence determination and analysis | Thermoclostridium stercorarium |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.20 | 6.2 | - |
cellobiose | - |
Thermoclostridium stercorarium |  |
| 2.4.1.49 | 0.04 | - |
cellotriose | - |
Thermoclostridium stercorarium | |
| 2.4.1.49 | 0.05 | - |
cellotetraose | - |
Thermoclostridium stercorarium | |
| 2.4.1.49 | 0.17 | - |
cellopentaose | - |
Thermoclostridium stercorarium | |
| 2.4.1.49 | 0.19 | - |
cellohexaose | - |
Thermoclostridium stercorarium | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.20 | 93000 | - |
SDS-PAGE, gel filtration | Thermoclostridium stercorarium |
| 2.4.1.49 | additional information | - |
N-terminal amino acid sequence | Thermoclostridium stercorarium |
| 2.4.1.49 | 91000 | - |
gel filtration | Thermoclostridium stercorarium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.20 | cellobiose + phosphate | Thermoclostridium stercorarium | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| 2.4.1.20 | cellobiose + phosphate | Thermoclostridium stercorarium NCIB 11754 | - |
alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| 2.4.1.49 | (1,4-beta-D-glucosyl)n + phosphate | Thermoclostridium stercorarium | involved in the catabolism of cellulose | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
| 2.4.1.49 | (1,4-beta-D-glucosyl)n + phosphate | Thermoclostridium stercorarium NCIB 11754 | involved in the catabolism of cellulose | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.20 | Thermoclostridium stercorarium | Q59316 | - |
- |
| 2.4.1.20 | Thermoclostridium stercorarium NCIB 11754 | Q59316 | - |
- |
| 2.4.1.49 | Thermoclostridium stercorarium | P77846 | - |
- |
| 2.4.1.49 | Thermoclostridium stercorarium | P77846 | gene CepB | - |
| 2.4.1.49 | Thermoclostridium stercorarium NCIB 11754 | P77846 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.20 | - |
Thermoclostridium stercorarium |
| 2.4.1.49 | - |
Thermoclostridium stercorarium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.20 | 4.7 | - |
purified enzyme | Thermoclostridium stercorarium |
| 2.4.1.49 | 9.2 | - |
purified enzyme, assay at 60°C and pH 6.0 | Thermoclostridium stercorarium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.20 | cellobiose + phosphate | - |
Thermoclostridium stercorarium | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| 2.4.1.20 | cellobiose + phosphate | - |
Thermoclostridium stercorarium NCIB 11754 | alpha-D-glucose 1-phosphate + D-glucose | - |
? | |
| 2.4.1.20 | additional information | only disaccharide accepted as substrate | Thermoclostridium stercorarium | ? | - |
? | |
| 2.4.1.20 | additional information | only disaccharide accepted as substrate | Thermoclostridium stercorarium NCIB 11754 | ? | - |
? | |
| 2.4.1.49 | (1,4-beta-D-glucosyl)n + phosphate | specific for oligosaccharides | Thermoclostridium stercorarium | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
| 2.4.1.49 | (1,4-beta-D-glucosyl)n + phosphate | involved in the catabolism of cellulose | Thermoclostridium stercorarium | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
| 2.4.1.49 | (1,4-beta-D-glucosyl)n + phosphate | specific for oligosaccharides | Thermoclostridium stercorarium NCIB 11754 | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
| 2.4.1.49 | (1,4-beta-D-glucosyl)n + phosphate | involved in the catabolism of cellulose | Thermoclostridium stercorarium NCIB 11754 | (1,4-beta-D-glucosyl)n-1 + alpha-D-glucose 1-phosphate | - |
? | |
| 2.4.1.49 | additional information | no phosphorolysis activity with cellobiose | Thermoclostridium stercorarium | ? | - |
? | |
| 2.4.1.49 | additional information | no phosphorolysis activity with cellobiose | Thermoclostridium stercorarium NCIB 11754 | ? | - |
? | |
| 2.4.1.49 | phosphate + cellohexaose | - |
Thermoclostridium stercorarium | cellopentaose + glucose 1-phosphate | - |
? | |
| 2.4.1.49 | phosphate + cellopentaose | - |
Thermoclostridium stercorarium | cellotetraose + glucose 1-phosphate | - |
? | |
| 2.4.1.49 | phosphate + cellotetraose | - |
Thermoclostridium stercorarium | cellotriose + glucose 1-phosphate | - |
? | |
| 2.4.1.49 | phosphate + cellotetraose | - |
Thermoclostridium stercorarium NCIB 11754 | cellotriose + glucose 1-phosphate | - |
? | |
| 2.4.1.49 | phosphate + cellotriose | - |
Thermoclostridium stercorarium | cellobiose + glucose 1-phosphate | - |
? | |
| 2.4.1.49 | phosphate + cellotriose | - |
Thermoclostridium stercorarium NCIB 11754 | cellobiose + glucose 1-phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 2.4.1.20 | monomer | 1 * 93000, SDS-PAGE, gel filtration | Thermoclostridium stercorarium |
| 2.4.1.49 | monomer | 1 * 91000, SDS-PAGE | Thermoclostridium stercorarium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.20 | 65 | - |
- |
Thermoclostridium stercorarium |
| 2.4.1.49 | 65 | - |
- |
Thermoclostridium stercorarium |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.20 | 60 | - |
stable for at least 42 h | Thermoclostridium stercorarium |
| 2.4.1.49 | 60 | - |
half-life: 30 h | Thermoclostridium stercorarium |
| 2.4.1.49 | 65 | - |
inactivation within 20 h | Thermoclostridium stercorarium |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.20 | 1.1 | - |
cellobiose | - |
Thermoclostridium stercorarium | |
| 2.4.1.49 | 2.7 | - |
cellotriose | - |
Thermoclostridium stercorarium | |
| 2.4.1.49 | 6.1 | - |
cellohexaose | - |
Thermoclostridium stercorarium | |
| 2.4.1.49 | 6.6 | - |
cellopentaose | - |
Thermoclostridium stercorarium | |
| 2.4.1.49 | 6.9 | - |
cellotetraose | - |
Thermoclostridium stercorarium | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.20 | 6 | 7 | - |
Thermoclostridium stercorarium |
| 2.4.1.49 | 6 | 7 | - |
Thermoclostridium stercorarium |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.20 | 5 | 8 | 50% activity | Thermoclostridium stercorarium |
| 2.4.1.49 | 5 | 8 | half-maximal activity at pH 5.0 and pH 8.0 | Thermoclostridium stercorarium |
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