BRENDA - Enzyme Database

UDPgalactose: ceramide galactosyltransferase from rat brain

Neskovic, N.M.; Mandel, P.; Gatt, S.; Methods Enzymol. 71, 521-527 (1981)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.47
phosphatidylcholine
stimulates
Rattus norvegicus
2.4.1.47
phosphatidylethanolamine
stimulates
Rattus norvegicus
2.4.1.47
Phospholipids
addition to incubation mixture containing Triton X-100 increases activity
Rattus norvegicus
General Stability
EC Number
General Stability
Organism
2.4.1.47
glycerol stabilizes
Rattus norvegicus
2.4.1.47
in presence of glycerol the enzyme can be frozen and rethawed several times without appreciable loss of stability
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.47
Detergents
inhibition by excess detergent
Rattus norvegicus
2.4.1.47
DL-sphingosine
-
Rattus norvegicus
2.4.1.47
Octanoyl-D-threo-p-nitro-1-phenyl-2-amino-1,3-propanediol
-
Rattus norvegicus
2.4.1.47
Protein inhibitor
from brain, kidney, spleen and liver of rat and other animals
Rattus norvegicus
2.4.1.47
sodium deoxycholate
strong inhibition
Rattus norvegicus
2.4.1.47
sodium taurocholate
strong inhibition
Rattus norvegicus
2.4.1.47
sodium taurodeoxycholate
strong inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.47
0.018
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
2.4.1.47
0.027
0.034
UDPgalactose
-
Rattus norvegicus
2.4.1.47
0.15
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.47
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.47
400000
500000
lipoprotein complex
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.47
Rattus norvegicus
-
15-20 days old
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.47
lipoprotein
-
Rattus norvegicus
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.47
-
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.47
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.47
0.002733
-
-
Rattus norvegicus
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.47
-20°C, 50% w/v glycerol, 1 month, about 30% loss of activity
Rattus norvegicus
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.47
additional information
not: glucosylceramide, lactosylceramide, asialo GM2ganglioside
489216
Rattus norvegicus
?
-
-
-
-
2.4.1.47
additional information
not: ceramide containing nonhydroxy fatty acids
489216
Rattus norvegicus
?
-
-
-
-
2.4.1.47
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
cerebroside
489216
Rattus norvegicus
?
2.4.1.47
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
2-hydroxy fatty acid cerebroside
489216
Rattus norvegicus
?
2.4.1.47
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
i.e. 2-hydroxy fatty acid galactosylceramide
489216
Rattus norvegicus
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.47
27
-
assay at
Rattus norvegicus
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.47
7.8
8.2
-
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.47
phosphatidylcholine
stimulates
Rattus norvegicus
2.4.1.47
phosphatidylethanolamine
stimulates
Rattus norvegicus
2.4.1.47
Phospholipids
addition to incubation mixture containing Triton X-100 increases activity
Rattus norvegicus
General Stability (protein specific)
EC Number
General Stability
Organism
2.4.1.47
glycerol stabilizes
Rattus norvegicus
2.4.1.47
in presence of glycerol the enzyme can be frozen and rethawed several times without appreciable loss of stability
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.47
Detergents
inhibition by excess detergent
Rattus norvegicus
2.4.1.47
DL-sphingosine
-
Rattus norvegicus
2.4.1.47
Octanoyl-D-threo-p-nitro-1-phenyl-2-amino-1,3-propanediol
-
Rattus norvegicus
2.4.1.47
Protein inhibitor
from brain, kidney, spleen and liver of rat and other animals
Rattus norvegicus
2.4.1.47
sodium deoxycholate
strong inhibition
Rattus norvegicus
2.4.1.47
sodium taurocholate
strong inhibition
Rattus norvegicus
2.4.1.47
sodium taurodeoxycholate
strong inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.47
0.018
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
2.4.1.47
0.027
0.034
UDPgalactose
-
Rattus norvegicus
2.4.1.47
0.15
-
2-(2-hydroxyacyl)sphingosine
Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.47
microsome
-
Rattus norvegicus
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.47
400000
500000
lipoprotein complex
Rattus norvegicus
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.47
lipoprotein
-
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.47
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.4.1.47
brain
-
Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.4.1.47
0.002733
-
-
Rattus norvegicus
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.47
-20°C, 50% w/v glycerol, 1 month, about 30% loss of activity
Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.47
additional information
not: glucosylceramide, lactosylceramide, asialo GM2ganglioside
489216
Rattus norvegicus
?
-
-
-
-
2.4.1.47
additional information
not: ceramide containing nonhydroxy fatty acids
489216
Rattus norvegicus
?
-
-
-
-
2.4.1.47
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
cerebroside
489216
Rattus norvegicus
?
2.4.1.47
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
2-hydroxy fatty acid cerebroside
489216
Rattus norvegicus
?
2.4.1.47
UDPgalactose + 2-(2-hydroxyacyl)sphingosine
i.e. 2-hydroxy fatty acid ceramide
489216
Rattus norvegicus
1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP
i.e. 2-hydroxy fatty acid galactosylceramide
489216
Rattus norvegicus
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.4.1.47
27
-
assay at
Rattus norvegicus
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.47
7.8
8.2
-
Rattus norvegicus