Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Neskovic, N.M.; Mandel, P.; Gatt, S.
    UDPgalactose: ceramide galactosyltransferase from rat brain (1981), Methods Enzymol., 71, 521-527.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.47 phosphatidylcholine stimulates Rattus norvegicus
2.4.1.47 phosphatidylethanolamine stimulates Rattus norvegicus
2.4.1.47 Phospholipids addition to incubation mixture containing Triton X-100 increases activity Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.1.47 glycerol stabilizes Rattus norvegicus
2.4.1.47 in presence of glycerol the enzyme can be frozen and rethawed several times without appreciable loss of stability Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.47 Detergents inhibition by excess detergent Rattus norvegicus
2.4.1.47 DL-sphingosine
-
 Rattus norvegicus
2.4.1.47 Octanoyl-D-threo-p-nitro-1-phenyl-2-amino-1,3-propanediol
-
 Rattus norvegicus
2.4.1.47 Protein inhibitor from brain, kidney, spleen and liver of rat and other animals Rattus norvegicus
2.4.1.47 sodium deoxycholate strong inhibition Rattus norvegicus
2.4.1.47 sodium taurocholate strong inhibition Rattus norvegicus
2.4.1.47 sodium taurodeoxycholate strong inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.47 0.018
-
 2-(2-hydroxyacyl)sphingosine Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM Rattus norvegicus
2.4.1.47 0.027 0.034 UDPgalactose
-
 Rattus norvegicus
2.4.1.47 0.15
-
 2-(2-hydroxyacyl)sphingosine Km value for ceramide is influenced by its dispersion state, in presence of 2 mM Triton S-100 biphasic kinetics are observed with increasing ceramide concentration, yielding two apparent Km values of 0.018 and 0.15 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.47 microsome
-
 Rattus norvegicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.47 400000 500000 lipoprotein complex Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.47 Rattus norvegicus
-
 15-20 days old
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.47 lipoprotein
-
 Rattus norvegicus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.47
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.47 brain
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.47 0.002733
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.1.47 -20°C, 50% w/v glycerol, 1 month, about 30% loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.47 additional information not: glucosylceramide, lactosylceramide, asialo GM2ganglioside Rattus norvegicus ?
-
 ?
2.4.1.47 additional information not: ceramide containing nonhydroxy fatty acids Rattus norvegicus ?
-
 ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP cerebroside ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP 2-hydroxy fatty acid cerebroside ?
2.4.1.47 UDPgalactose + 2-(2-hydroxyacyl)sphingosine i.e. 2-hydroxy fatty acid ceramide Rattus norvegicus 1-(beta-D-galactosyl)-2-(2-hydroxyacyl)sphingosine + UDP i.e. 2-hydroxy fatty acid galactosylceramide ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.47 27
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.47 7.8 8.2
-
 Rattus norvegicus