BRENDA - Enzyme Database

Studies of phospholipid-requiring bacterial enzymes. III. Purification and properties of uridine diphosphate glucose:lipopolysaccharide glucosyltransferase I

Muller, E.; Hinckley, A.; Rothfield, L.; J. Biol. Chem. 247, 2614-2622 (1972)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.44
Phospholipid
phosphatidylethanolamine most effective; required for activity
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
phosphatidylethanolamine
requirement, most effective phospholipid, phosphatidylethanolamine containing unsaturated or cyclopropane acyl groups is more effective than that containing saturated acyl groups
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Phospholipid
requirement
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.58
iodoacetamide
5 mM, weak
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
additional information
not inhibited by p-chloromercuribenzoate, no detectable inhibition by non-substrate lipopolysaccharides obtained from other mutant strains of Salmonella typhimurium: G30G, G30A, G30, TV119, LT2
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.58
0.03
-
lipopolysaccharide
in presence of optimal concentrations of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
0.1
-
lipopolysaccharide
in absence of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
0.33
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.44
membrane
bound
Salmonella enterica subsp. enterica serovar Typhimurium
16020
-
2.4.1.58
membrane
-
Salmonella enterica subsp. enterica serovar Typhimurium
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.58
Ba2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Ca2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Co2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Mg2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.58
20000
-
several peaks suggesting aggregation of enzyme, sucrose density gradient centrifugation
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.44
UDP-galactose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium G30A
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium LT2
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
ir
2.4.1.58
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
UDP + D-glucosyllipopolysaccharide
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.4.1.44
Salmonella enterica subsp. enterica serovar Typhimurium
-
LT2
-
2.4.1.44
Salmonella enterica subsp. enterica serovar Typhimurium LT2
-
LT2
-
2.4.1.58
Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-
2.4.1.58
Salmonella enterica subsp. enterica serovar Typhimurium G30A
-
-
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.58
additional information
no detectable phospholipid or lipopolysaccharide in the purified enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary)
EC Number
Commentary
Organism
2.4.1.44
partial
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.44
0.00284
-
purified enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability
EC Number
Storage Stability
Organism
2.4.1.58
4░C, 20% glycerol, for about 5 days: retains full activity, 10% loss of activity per week thereafter
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.44
UDP-galactose + lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
-
ir
2.4.1.58
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
2.4.1.58
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
-
-
-
-
2.4.1.58
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
-
-
-
2.4.1.58
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
2.4.1.58
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
2.4.1.58
UDPglucose + lipopolysaccharide
glucose is transferred only to the glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032, a strain lacking the glucosyl transferase system, transfers glucose to the L-glycero-D-mannoheptosyl residue, maximum incorporation of 0.5 mol glucose per mol of heptose
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
2.4.1.58
UDPglucose + lipopolysaccharide
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
2.4.1.58
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
2.4.1.58
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
2.4.1.58
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
2.4.1.58
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
Subunits
EC Number
Subunits
Commentary
Organism
2.4.1.58
monomer
1 * 20000, additional faint bands: 44,5, 49 and 72 kDa, probably a single polypeptide chain, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [░C]
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.4.1.58
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.58
7.5
8.5
Tris-maleate or Tris-Cl buffers
Salmonella enterica subsp. enterica serovar Typhimurium
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.4.1.44
Phospholipid
phosphatidylethanolamine most effective; required for activity
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
phosphatidylethanolamine
requirement, most effective phospholipid, phosphatidylethanolamine containing unsaturated or cyclopropane acyl groups is more effective than that containing saturated acyl groups
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Phospholipid
requirement
Salmonella enterica subsp. enterica serovar Typhimurium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
2.4.1.58
iodoacetamide
5 mM, weak
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
additional information
not inhibited by p-chloromercuribenzoate, no detectable inhibition by non-substrate lipopolysaccharides obtained from other mutant strains of Salmonella typhimurium: G30G, G30A, G30, TV119, LT2
Salmonella enterica subsp. enterica serovar Typhimurium
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.4.1.58
0.03
-
lipopolysaccharide
in presence of optimal concentrations of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
0.1
-
lipopolysaccharide
in absence of phosphatidylethanolamine
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
0.33
-
UDPglucose
-
Salmonella enterica subsp. enterica serovar Typhimurium
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.4.1.44
membrane
bound
Salmonella enterica subsp. enterica serovar Typhimurium
16020
-
2.4.1.58
membrane
-
Salmonella enterica subsp. enterica serovar Typhimurium
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.4.1.58
Ba2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Ca2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Co2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
Mg2+
requires divalent cation, in decreasing order of effectiveness: Mg2+, Ba2+, Ca2+, Co2+, relative activities: Mg2+: 1.0, Ba2+: 0.77, Ca2+: 0.65, Co2+: 0.31
Salmonella enterica subsp. enterica serovar Typhimurium
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.4.1.58
20000
-
several peaks suggesting aggregation of enzyme, sucrose density gradient centrifugation
Salmonella enterica subsp. enterica serovar Typhimurium
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.4.1.44
UDP-galactose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium G30A
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium LT2
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
ir
2.4.1.58
UDPglucose + lipopolysaccharide
Salmonella enterica subsp. enterica serovar Typhimurium
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
UDP + D-glucosyllipopolysaccharide
-
-
?
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
2.4.1.58
additional information
no detectable phospholipid or lipopolysaccharide in the purified enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.4.1.44
partial
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
-
Salmonella enterica subsp. enterica serovar Typhimurium
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.4.1.44
0.00284
-
purified enzyme
Salmonella enterica subsp. enterica serovar Typhimurium
2.4.1.58
additional information
-
-
Salmonella enterica subsp. enterica serovar Typhimurium
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
2.4.1.58
4░C, 20% glycerol, for about 5 days: retains full activity, 10% loss of activity per week thereafter
Salmonella enterica subsp. enterica serovar Typhimurium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.4.1.44
UDP-galactose + lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
-
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
ir
2.4.1.44
UDP-galactose + lipopolysaccharide
transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide
-
-
-
ir
2.4.1.58
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
-
-
-
-
2.4.1.58
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
-
-
-
-
2.4.1.58
additional information
poor sugar donor: UDPgalactose, no sugar donors: TDPglucose, CDPglucose, ADPglucose, GDPmannose, glucose, glucose-1-phosphate, galactose, alpha-methylglucoside, beta-methylglucoside, no sugar acceptors: lipopolysaccharides from Salmonella typhimurium G30A, G30G, G30, TV119, LT2
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
-
-
-
-
2.4.1.58
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
2.4.1.58
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
2.4.1.58
UDPglucose + lipopolysaccharide
glucose is transferred only to the glucose-deficient lipopolysaccharide obtained from Salmonella typhimurium SL1032, a strain lacking the glucosyl transferase system, transfers glucose to the L-glycero-D-mannoheptosyl residue, maximum incorporation of 0.5 mol glucose per mol of heptose
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium
?
2.4.1.58
UDPglucose + lipopolysaccharide
addition of glucose during the biosynthesis of the lipopolysaccharide component of the cell envelope
489212
Salmonella enterica subsp. enterica serovar Typhimurium
UDP + D-glucosyllipopolysaccharide
-
-
-
?
2.4.1.58
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
2.4.1.58
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium G30A
?
2.4.1.58
UDPglucose + lipopolysaccharide
core region of the lipopolysaccharide
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
2.4.1.58
UDPglucose + lipopolysaccharide
UDPglucose is the only effective glucosyl donor
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
UDP + D-glucosyllipopolysaccharide
-
489212
Salmonella enterica subsp. enterica serovar Typhimurium LT2
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.4.1.58
monomer
1 * 20000, additional faint bands: 44,5, 49 and 72 kDa, probably a single polypeptide chain, SDS-PAGE
Salmonella enterica subsp. enterica serovar Typhimurium
Temperature Optimum [░C] (protein specific)
EC Number
Temperature Optimum [░C]
Temperature Optimum Maximum [░C]
Commentary
Organism
2.4.1.58
37
-
assay at
Salmonella enterica subsp. enterica serovar Typhimurium
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.4.1.58
7.5
8.5
Tris-maleate or Tris-Cl buffers
Salmonella enterica subsp. enterica serovar Typhimurium