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Literature summary extracted from
- Studies of a phospholipid-requiring bacterial enzyme. I. Purification and properties of uridine diphosphate galactose: lipopolysaccharide alpha-3-galactosyl transferase (1969), Biochemistry, 8, 3500-3507.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.44 | Phospholipid | required for activity | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 2.4.1.44 | Phospholipid | effect on several strains, overview | Salmonella enterica subsp. enterica serovar Typhimurium | |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 2.4.1.44 | one cycle of freezing and thawing causes marked loss of activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.44 | Nonsubstrate lipopolysaccharide | competitive, Salmonella typhimurium SL1032, TV119, LT2 lipopolysaccharides; effect on several strains, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 2.4.1.44 | 0.074 | - |
UDP-galactose | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 2.4.1.44 | CaCl2 | no activity in absence of divalent cations, 20% of the activation with MgCl2 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 2.4.1.44 | MgCl2 | no activity in absence of divalent cations, highest activity with MgCl2 | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 2.4.1.44 | MnCl2 | no activity in absence of divalent cations, 20% of the activation with MgCl2 | Salmonella enterica subsp. enterica serovar Typhimurium | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 2.4.1.44 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
G-30A | - |
| 2.4.1.44 | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | - |
G-30A | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 2.4.1.44 | additional information | unidentified lipid-soluble component which contains no phosphorus is bound to the enzyme, it causes aggregation of the enzyme, but no role of the lipid in enzyme reaction has yet been established | Salmonella enterica subsp. enterica serovar Typhimurium |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 2.4.1.44 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.44 | additional information | - |
several bacterial strains, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
| 2.4.1.44 | 0.00048 | - |
supernatant fraction | Salmonella enterica subsp. enterica serovar Typhimurium |
| 2.4.1.44 | 0.0015 | - |
purified enzyme | Salmonella enterica subsp. enterica serovar Typhimurium |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 2.4.1.44 | 4°C, pH 6.8, 0.02-0.04 mg/ml protein, stable for at least 1 month | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060 | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | highly specific for UDPgalactose | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | Salmonella enterica subsp. enterica serovar Typhimurium | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | lipopolysaccharides: lacking the alpha-3-galactosyl residue of the core lipopolysaccharide, from Salmonella typhimurium strains G-30 and SL1060 | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | highly specific for UDPgalactose | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | transfers D-galactosyl residues to D-glucose in the partially completed core of lipopolysaccharide | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
| 2.4.1.44 | UDP-galactose + lipopolysaccharide | catalyzes one of the reactions involved in biosynthesis of cell envelope lipopolysaccharide of the organism | Salmonella enterica subsp. enterica serovar Typhimurium G-30A | UDP + 1,3-alpha-D-galactosyl-lipopolysaccharide | - |
ir | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.44 | 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.44 | 8.5 | 9 | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 2.4.1.44 | 8.5 | - |
4°C, several days, inactivation | Salmonella enterica subsp. enterica serovar Typhimurium |
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