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Literature summary extracted from

  • Takeuchi, Y.; Tsumuraya, Y.
    In vitro biosynthesis of homogalacturonan by a membrane-bound galacturonosyltransferase from epicotyls of azuki bean (2001), Biosci. Biotechnol. Biochem., 65, 1519-1527.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.43 Triton X-100 0.5% acceptor acid-soluble polygalacturonic acid, 170% increase in activity Vigna angularis
2.4.1.43 Triton X-102 0.5%, acceptor acid-soluble polygalacturonic acid, 190% increase in activity Vigna angularis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.43 ADP 10 mM, 60% inhibition Vigna angularis
2.4.1.43 ATP 10 mM, 83% inhibition Vigna angularis
2.4.1.43 dithiothreitol 10 mM, 61% inhibition Vigna angularis
2.4.1.43 EDTA 10 mM, 82% inhibition Vigna angularis
2.4.1.43 EGTA 10 mM, 96% inhibition Vigna angularis
2.4.1.43 GTP 10 mM, 80% inhibition Vigna angularis
2.4.1.43 NaF 10 mM, 56% inhibition Vigna angularis
2.4.1.43 S-adenosyl-L-methionine 2 mM, 10% inhibition Vigna angularis
2.4.1.43 UTP 10 mM, 86% inhibition Vigna angularis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.43 additional information
-
 additional information Km for acid-soluble polygalacturonic acid of 10000 Da: 0.02 mg/ml Vigna angularis
2.4.1.43 0.14
-
 UDP-D-galacturonic acid
-
 Vigna angularis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.43 membrane
-
 Vigna angularis 16020
-
2.4.1.43 microsome
-
 Vigna angularis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.43 MnCl2 5 mM, 40fold stimulation Vigna angularis
2.4.1.43 Zn2+ 5 mM, 8fold stimulation Vigna angularis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.43 Vigna angularis
-
 azuki bean
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.43 seedling
-
 Vigna angularis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.43 0.0013 0.002 activity in microsomes Vigna angularis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.43 UDP-D-galacturonic acid + (1,4-alpha-D-galacturonosyl)n acid-soluble polygalacturonic acid of 10000 Da is a better acceptor than acid-insoluble polygalacturonic acid of 70000 Da Vigna angularis UDP + (1,4-alpha-D-galacturonosyl)n+1
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.43 25 35 in the presence of 5 mM Mn2+ and 0.5% Triton X-100 Vigna angularis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.43 30
-
 90 min, 505 loss of activity Vigna angularis
2.4.1.43 45
-
 90 min, complete loss of activity Vigna angularis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.43 6.8 7.8 in the presence of 5 mM Mn2+ and 0.5% Triton X-100 Vigna angularis